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Bacterial Vaccines

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2414)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates Against Shigella flexneri

    • Chiuan Yee Leow, Candy Chuah, Abu Bakar Abdul Majeed, Norazmi Mohd Nor, Chiuan Herng Leow
    Pages 17-35
  3. Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines

    • Jasmin Fertey, Bastian Standfest, Jana Beckmann, Martin Thoma, Thomas Grunwald, Sebastian Ulbert
    Pages 97-113
  4. Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria

    • Somshukla Chaudhuri, Nikolas F. Ewasechko, Luisa Samaniego-Barron, Jamie E. Fegan, Anthony B. Schryvers
    Pages 115-140
  5. Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery

    • Lili Zhao, Sahra Bashiri, Istvan Toth, Mariusz Skwarczynski
    Pages 141-149
  6. Methods for Assessment of OMV/GMMA Quality and Stability

    • Francesca Micoli, Renzo Alfini, Carlo Giannelli
    Pages 227-279
  7. Production of Vaccines Using Biological Conjugation

    • Emily J. Kay, Vanessa S. Terra
    Pages 281-300
  8. Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines Against Bacterial Pathogens

    • Anneliese S. Ashhurst, Cameron C. Hanna, Richard J. Payne, Warwick J. Britton
    Pages 301-323
  9. Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma

    • Frances Alexander, Emily Brunt, Holly Humphries, Breeze Cavell, Stephanie Leung, Lauren Allen et al.
    Pages 341-362
  10. Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets

    • Evgeny A. Semchenko, Freda E.-C. Jen, Michael P. Jennings, Kate L. Seib
    Pages 363-372

About this book

The importance of vaccines to combat bacterial diseases cannot be overstated. Methods used in the development and testing of these vaccines are evolving rapidly as a direct consequence of the availability of advanced technologies. This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use. Chapters cover in silico analytical methods such as reverse vaccinology and machine learning; low-energy electron irradiation for the generation of inactivated bacterial vaccines; methods for assessment of OMV/GMMA quality and stability; and controlled human infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Bacterial Vaccines: Methods and Protocols is a valuable resource for novice and expert researchers interested in learning more about this important and constantly evolving field.  



Editors and Affiliations

  • Department of Infectious Disease, Imperial College London, London, UK

    Fadil Bidmos, Janine Bossé, Paul Langford

Bibliographic Information

  • Book Title: Bacterial Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Fadil Bidmos, Janine Bossé, Paul Langford

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1900-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1899-8Published: 17 November 2021

  • Softcover ISBN: 978-1-0716-1902-5Published: 18 November 2022

  • eBook ISBN: 978-1-0716-1900-1Published: 16 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 453

  • Number of Illustrations: 21 b/w illustrations, 89 illustrations in colour

  • Topics: Microbiology, Virology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access