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HIV Reservoirs

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Guido Poli

    1. School of Medicine and Human Immuno-Virology (H.I.V.) Group, San Raffaele University and Scientific Institute, Milano, Italy

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  2. Elisa Vicenzi

    1. Viral Pathogenesis and Biosafety Group, San Raffaele Scientific Institute, Milano, Italy

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  3. Fabio Romerio

    1. Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2407)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Cell Lines with Minimal but Inducible Proviral Expression

    1. Front Matter

      Pages 1-1
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    2. Jurkat-Derived (J-Lat, J1.1, and Jurkat E4) and CEM-Derived T Cell Lines (8E5 and ACH-2) as Models of Reversible Proviral Latency

      • Anthony Rodari, Guido Poli, Carine Van Lint
      Pages 3-15

    3. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models of Reversible Proviral Latency

      • Guido Poli
      Pages 17-28

  3. In Vitro and Ex Vivo Primary Cell Models of Minimal but Inducible Proviral Expression

    1. Front Matter

      Pages 29-29
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    2. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency in Primary Human CD4+ T Cells

      • Rui Li, Fabio Romerio
      Pages 31-43

    3. The Cultured TCM Model of HIV Latency

      • Alberto Bosque
      Pages 45-55

    4. A Reliable Primary Cell Model for HIV Latency: The QUECEL (Quiescent Effector Cell Latency) Method

      • Meenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, Jonathan Karn
      Pages 57-68

    5. TGF-β Signaling Supports HIV Latency in a Memory CD4+ T Cell Based In Vitro Model

      • Sydney Bergstresser, Deanna A. Kulpa
      Pages 69-79

    6. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoir Quantification

      • Amélie Cattin, Augustine Fert, Delphine Planas, Petronela Ancuta
      Pages 81-89

    7. Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF)

      • Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, Carlo Federico Perno
      Pages 91-96

    8. Human Monocyte-Derived Macrophages (MDM): Model 2

      • Francesca Graziano, Elisa Vicenzi, Guido Poli
      Pages 97-101

    9. Modeling HIV Latency in Astrocytes with the Human Neural Progenitor Cell Line HNSC.100

      • Amelie Bauer, Ruth Brack-Werner
      Pages 103-114

    10. Hematopoietic Stem and Progenitor Cells (HSPCs)

      • Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, Kathleen L. Collins
      Pages 115-154

  4. In Vitro and Ex Vivo Tissue-Derived Models of Reversible Proviral Latency

    1. Front Matter

      Pages 155-155
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    2. Ex Vivo HIV Infection Model of Cervico-Vaginal and Rectal Tissue

      • Louise A. Ouattara, Nikolas C. Vann, Gustavo F. Doncel
      Pages 157-172

    3. More than a Gender Issue: Testis as a Distinctive HIV Reservoir and Its Implication for Viral Eradication

      • Jean-Pierre Routy, Franck P. Dupuy, John Lin, Stéphane Isnard
      Pages 173-186

  5. Animal Models

    1. Front Matter

      Pages 187-187
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    2. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells

      • Fernando Real, Yonatan Ganor, Morgane Bomsel
      Pages 189-204

    3. Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond

      • Nabila Jabrane-Ferrat, Hicham El Costa
      Pages 205-213
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Keywords

About this book

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.

Editors and Affiliations

  • School of Medicine and Human Immuno-Virology (H.I.V.) Group, San Raffaele University and Scientific Institute, Milano, Italy

    Guido Poli

  • Viral Pathogenesis and Biosafety Group, San Raffaele Scientific Institute, Milano, Italy

    Elisa Vicenzi

  • Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, USA

    Fabio Romerio

Bibliographic Information

  • Book Title: HIV Reservoirs

  • Book Subtitle: Methods and Protocols

  • Editors: Guido Poli, Elisa Vicenzi, Fabio Romerio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1871-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1870-7Published: 06 January 2022

  • Softcover ISBN: 978-1-0716-1873-8Published: 07 January 2023

  • eBook ISBN: 978-1-0716-1871-4Published: 05 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 451

  • Number of Illustrations: 61 b/w illustrations

  • Topics: Infectious Diseases

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