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Insoluble Proteins

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Elena Garcia Fruitós

    1. Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Anna Arís Giralt

    1. Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2406)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Recombinant Protein Production and Purification of Insoluble Proteins

    • Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Elena Garcia-Fruitós
    Pages 1-31

  3. Prokaryotic Expression Systems

    1. Front Matter

      Pages 33-33
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    2. Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

      • Claudia Ortega, Pablo Oppezzo, Agustín Correa
      Pages 35-64

    3. A3D 2.0 Update for the Prediction and Optimization of Protein Solubility

      • Jordi Pujols, Valentín Iglesias, Jaime Santos, Aleksander Kuriata, Sebastian Kmiecik, Salvador Ventura
      Pages 65-84

    4. Tailoring Codon Usage to the Underlying Biology for Protein Expression Optimization

      • Zahra Alirezaeizanjani, Jan-Hendrik Trösemeier, Christel Kamp, Sophia Rudorf
      Pages 85-92

    5. Protein Expression Optimization Strategies in E. coli: A Tailored Approach in Strain Selection and Parallelizing Expression Conditions

      • Shyn Ric Tang, Balaji Somasundaram, Linda H. L. Lua
      Pages 93-111

    6. A Novel Approach for the Production of Aggregation-Prone Proteins Using the Spidroin-Derived NT* Tag

      • Nina Kronqvist, Anna Rising, Jan Johansson
      Pages 113-130

    7. Cleavable Self-Aggregating Tags (cSAT) for Therapeutic Peptide Expression and Purification

      • Xiaofeng Yang, Zhanglin Lin, Yanyun Jing
      Pages 131-143

    8. Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility

      • Shuxiong Chen, Bernd Rehm
      Pages 145-153

    9. Introduction of a Hexalysine (6 K) Tag Can Protect from N-Terminal Cleavage and Increase Yield of Recombinant Proteins Expressed in the Periplasm of E. coli

      • Vasiliki Paraskevopoulou, Mohammed Alissa, Naim Hage, Franco H. Falcone
      Pages 155-167

    10. Two-Tiered Selection and Screening Strategy to Increase Functional Enzyme Production in E. coli

      • Jason T. Boock, May Taw, Brian C. King, Robert J. Conrado, Donna M. Gibson, Matthew P. DeLisa
      Pages 169-187

    11. Method for Production of Cysteine-Rich Proteins in Lactococcus lactis Expression System

      • Susheel K. Singh, Vandana Singh
      Pages 189-203

    12. Heterologous Protein Production in Lactobacillus (plantarum) Using pSIP Vectors

      • Geir Mathiesen, Lars Axelsson, Vincent G. H. Eijsink
      Pages 205-217

    13. Soluble Recombinant Protein Production in Pseudoalteromonas haloplanktis TAC125: The Case Study of the Full-Length Human CDKL5 Protein

      • Marzia Calvanese, Andrea Colarusso, Concetta Lauro, Ermenegilda Parrilli, Maria Luisa Tutino
      Pages 219-232

    14. A Protocol to Enhance Soluble Protein Expression in the Cytoplasm of Bacillus subtilis

      • Hoang D. Nguyen, Trang T. P. Phan
      Pages 233-243

    15. Applications of Cell-Free Synthesized Membrane Protein Precipitates

      • Julija Mezhyrova, Karsten Mörs, Clemens Glaubitz, Volker Dötsch, Frank Bernhard
      Pages 245-266

  4. Eukaryotic Expression Systems

    1. Front Matter

      Pages 267-267
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    2. High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins

      • Yasuaki Kawarasaki, Takeshi Kurose, Sayaka Ohashi, Runa Watabe, Mizuki Tanaka, Keisuke Ito
      Pages 269-279

    3. Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control

      • Simon Pichard, Nathalie Troffer-Charlier, Isabelle Kolb-Cheynel, Pierre Poussin-Courmontagne, Wassim Abdulrahman, Catherine Birck et al.
      Pages 281-317
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Keywords

About this book

This volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Insoluble Proteins: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

Editors and Affiliations

  • Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain

    Elena Garcia Fruitós, Anna Arís Giralt

Bibliographic Information

  • Book Title: Insoluble Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Elena Garcia Fruitós, Anna Arís Giralt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1859-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1858-5Published: 29 January 2022

  • Softcover ISBN: 978-1-0716-1861-5Published: 29 January 2022

  • eBook ISBN: 978-1-0716-1859-2Published: 28 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 533

  • Number of Illustrations: 38 b/w illustrations, 60 illustrations in colour

  • Topics: Protein Science

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