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Membrane Lipids

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Charles G. Cranfield

    1. School of Life Sciences, University of Technology Sydney, Ultimo, Australia

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2402)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Methods for Forming Giant Unilamellar Fatty Acid Vesicles

    • Lauren A. Lowe, Daniel W. K. Loo, Anna Wang
    Pages 1-12

  3. Preparing Ion Channel Switch Membrane-Based Biosensors

    • Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, Stella M. Valenzuela
    Pages 13-20

  4. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer

    • Charles G. Cranfield, Anton P. Le Brun, Alvaro Garcia, Bruce A. Cornell, Stephen A. Holt
    Pages 21-30

  5. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes

    • Tadas Penkauskas, Filipas Ambrulevičius, Gintaras Valinčius
    Pages 31-59

  6. Measuring Voltage–Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes

    • Hadeel Alobeedallah, Bruce A. Cornell, Hans Coster
    Pages 61-69

  7. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)

    • Hadeel Alobeedallah, Bruce A. Cornell, Hans Coster
    Pages 71-79

  8. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

    • Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, Charles G. Cranfield
    Pages 81-92

  9. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

    • Kan Shoji
    Pages 93-102

  10. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations

    • Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, Evelyne Deplazes
    Pages 103-121

  11. Establishing a Lipid Bilayer for Molecular Dynamics Simulations

    • Robby Manrique
    Pages 123-130

  12. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins

    • Amanda Buyan, Ben Corry
    Pages 131-141

  13. Small-Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling

    • Kathleen Wood
    Pages 143-149

  14. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation

    • Michael H.  L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, Drew Marquardt
    Pages 151-161

  15. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

    • Mitchell DiPasquale, Michael H.  L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, Drew Marquardt
    Pages 163-177

  16. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers

    • Stephen A. Holt, Tara E. Oliver, Andrew R. J. Nelson
    Pages 179-197

  17. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs)

    • Kinga Burdach, Damian Dziubak, Slawomir Sek
    Pages 199-207

  18. Manipulation of Lipid Membranes with Thermal Stimuli

    • Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, Irep Gözen
    Pages 209-225

  19. Analyzing Morphological Properties of Early-Stage Toxic Amyloid β Oligomers by Atomic Force Microscopy

    • Dusan Mrdenovic, Jacek Lipkowski, Piotr Pieta
    Pages 227-241

  20. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains

    • Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, Sabina M. Maté
    Pages 243-256
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Keywords

About this book

This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.

Editors and Affiliations

  • School of Life Sciences, University of Technology Sydney, Ultimo, Australia

    Charles G. Cranfield

Bibliographic Information

  • Book Title: Membrane Lipids

  • Book Subtitle: Methods and Protocols

  • Editors: Charles G. Cranfield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1843-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1842-4Published: 02 December 2021

  • Softcover ISBN: 978-1-0716-1845-5Published: 03 December 2021

  • eBook ISBN: 978-1-0716-1843-1Published: 01 December 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 300

  • Number of Illustrations: 9 b/w illustrations, 136 illustrations in colour

  • Topics: Cell Biology, Biological and Medical Physics, Biophysics, Lipidology

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