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Peptide Macrocycles

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Matthew B. Coppock

    1. DEVCOM Army Research Lab, Adelphi, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Alexander J. Winton

    1. DEVCOM Army Research Lab, Adelphi, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2371)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Peptide Macrocycle Synthesis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Three Methods for Peptide Cyclization Via Lactamization

      • Laura Posada, Gloria Serra
      Pages 3-17

    3. Solid-Phase Peptide Cyclization with Two Disulfide Bridges

      • Da’san M. M. Jaradat
      Pages 19-29

    4. Peptide Macrocyclization Through Palladium-Catalyzed Late-Stage C-H Activation

      • Chuangxu Cai, Shu Liu, Huan Wang
      Pages 31-42

    5. Three Methods for the Solution Phase Synthesis of Cyclic Peptides

      • Angelika Ullrich, Lukas Junk, Uli Kazmaier
      Pages 43-61

    6. Computational Design of Structured and Functional Peptide Macrocycles

      • Stephanie Berger, Parisa Hosseinzadeh
      Pages 63-100

    7. On-Resin Peptide Cyclization Using the 3-Amino-4-(Methylamino)Benzoic Acid MeDbz Linker

      • Bengt H. Gless, Christian A. Olsen
      Pages 101-115

    8. Scalable and Efficient In Planta Biosynthesis of Sunflower Trypsin Inhibitor-1 (SFTI) Peptide Therapeutics

      • Thomas N. G. Handley, Mark A. Jackson, David J. Craik
      Pages 117-142

    9. Ligation, Macrocyclization, and Simultaneous Functionalization of Peptides by Multicomponent Reactions (MCR)

      • Aldrin V. Vasco, Manuel G. Ricardo, Daniel G. Rivera, Ludger A. Wessjohann
      Pages 143-157

    10. Diels-Alder Cycloadditions for Peptide Macrocycle Formation

      • Jeffrey E. Montgomery, Raymond E. Moellering
      Pages 159-174

  3. Peptide Macrocycle Combinatorial Library Synthesis and Screening

    1. Front Matter

      Pages 175-175
      Download chapter PDF

    2. Protein Catalyzed Capture (PCC) Agents for Antigen Targeting

      • Matthew N. Idso, Bert T. Lai, Heather D. Agnew, James R. Heath
      Pages 177-191

    3. Preparation of Bacterial Cell-Surface Displayed Semisynthetic Cyclic Peptides

      • Shubhendu Palei, Joachim Jose, Henning D. Mootz
      Pages 193-213

    4. The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultrahigh-Throughput Screening in Bacteria

      • Dafni C. Delivoria, Georgios Skretas
      Pages 215-246

    5. In Vitro Selection of Thioether-Closed Macrocyclic Peptide Ligands by Means of the RaPID System

      • Takayuki Katoh, Yuki Goto, Hiroaki Suga
      Pages 247-259

    6. MOrPH-PhD: A Phage Display System for the Functional Selection of Genetically Encoded Macrocyclic Peptides

      • Yu Gu, Jacob A. Iannuzzelli, Rudi Fasan
      Pages 261-286

    7. Discovery of Antimicrobial Peptide Macrocycles Through Bacterial Display

      • Justin R. Randall, Cory D. DuPai, Bryan W. Davies
      Pages 287-298

  4. Peptide Macrocycle Characterization

    1. Front Matter

      Pages 299-299
      Download chapter PDF

    2. Assessing the Cellular Uptake, Endosomal Escape , and Cytosolic Entry Efficiencies of Cyclic Peptides

      • Heba Salim, Dehua Pei
      Pages 301-316
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Keywords

About this book

This volume explores the latest techniques and strategies used to study the field of peptide macrocycles. The chapters in this book ae organized into four parts: macrocycles synthesis, combinational library synthesis and screening, macrocycle characterization, and unique applications. Part One looks at a variety of peptide cyclization methodologies, and Part Two describes methods for the creation of peptide macrocycles libraries and their subsequent screening against biological targets of interest. Part Three discusses the study and characterization of peptide macrocycle-target interactions, and Part Four introduces unique applications for peptide macrocycles, from higher-order structure formation to post-synthetic functional modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and comprehensive, Peptide Macrocycles: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this developing field.


Editors and Affiliations

  • DEVCOM Army Research Lab, Adelphi, USA

    Matthew B. Coppock, Alexander J. Winton

Bibliographic Information

  • Book Title: Peptide Macrocycles

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew B. Coppock, Alexander J. Winton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1689-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1688-8Published: 02 October 2021

  • Softcover ISBN: 978-1-0716-1691-8Published: 02 October 2022

  • eBook ISBN: 978-1-0716-1689-5Published: 01 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 469

  • Number of Illustrations: 23 b/w illustrations, 83 illustrations in colour

  • Topics: Biochemical Engineering

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