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Fe-S Proteins

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Patricia C. Dos Santos

    1. Department of Chemistry, Wake Forest University, Winston Salem, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2353)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Methods for Genetic Manipulation and Fe-S Protein Expression

    1. Front Matter

      Pages 1-1
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    2. Molecular Biology and Genetic Tools to Investigate Functional Redundancy Among Fe-S Cluster Carriers in E. coli

      • Yohann Duverger, Béatrice Py
      Pages 3-36

    3. Study of Fe-S Cluster Proteins in Methanococcus maripaludis , a Model Archaeal Organism

      • Cuiping Zhao, Christina A. Roberts, Ian J. Drake, Yuchen Liu
      Pages 37-50

    4. Genetic Approaches to Uncover Gene Products Involved in Iron-Sulfur Protein Maturation: High-Throughput Genomic Screening Using Transposon Sequencing

      • Valerie J. Carabetta, Karla Esquilin-Lebron, Ehud Zelzion, Jeffrey M. Boyd
      Pages 51-68

    5. Methods for Heterologous Overproduction of Fe-S Proteins

      • Elliot I. Corless, Edwin Antony
      Pages 69-78

    6. Enzymatic and Chemical In Vitro Reconstitution of Iron-Sulfur Cluster Proteins

      • Mauro Marengo, Rita Puglisi, Simonetta Oliaro-Bosso, Annalisa Pastore, Salvatore Adinolfi
      Pages 79-95

    7. Expression, Isolation, and Characterization of Vanadium Nitrogenase from Azotobacter vinelandii

      • Katharina Parison, Jakob Gies-Elterlein, Christian Trncik, Oliver Einsle
      Pages 97-121

  3. Biochemical Methods to Probe Steps Involved in the Synthesis of Fe-S Clusters

    1. Front Matter

      Pages 123-123
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    2. Ni-NTA Affinity Chromatography to Characterize Protein–Protein Interactions During Fe-S Cluster Biogenesis

      • Terrell D. Carter, F. Wayne Outten
      Pages 125-136

    3. tRNA Modifications as a Readout of S and Fe-S Metabolism

      • Ashley M. Edwards, Maame A. Addo, Patricia C. Dos Santos
      Pages 137-154

    4. Methods to Unravel the Roles of ATPases in Fe-S Cluster Biosynthesis

      • Christa N. Molé, Kinjal Dave, Deborah L. Perlstein
      Pages 155-171

    5. Methods to Investigate the Kinetic Profile of Cysteine Desulfurases

      • Maame A. Addo, Ashley M. Edwards, Patricia C. Dos Santos
      Pages 173-189

    6. A Fast and Ratiometric Method for Quantification of Cysteine-Bound Persulfides Based on Alkylation and Gel-Shift Assays

      • Sylvain Gervason, Batoul Srour, Benoit D’Autréaux
      Pages 191-205

  4. Biophysical Methods to Study Fe-S Clusters and Reactions Promoted by Metalloenzymes

    1. Front Matter

      Pages 207-207
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    2. Circular Dichroism to Probe the Synthesis, Transfer, and Stability of Fe-S Clusters

      • Stefania Iametti, Francesco Bonomi, Alberto Barbiroli
      Pages 209-229

    3. Native Mass Spectrometry of Iron-Sulfur Proteins

      • Jason C. Crack, Nick E. Le Brun
      Pages 231-258

    4. Characterization of Paramagnetic Iron-Sulfur Clusters Using Electron Paramagnetic Resonance Spectroscopy

      • Troy A. Stich
      Pages 259-280

    5. Characterization of Fe-S Clusters in Proteins by MÓ§ssbauer Spectroscopy

      • Chie Ueda, Michelle Langton, Maria-Eirini Pandelia
      Pages 281-305

    6. Biochemical Approaches to Probe the Role of the Auxiliary Iron-Sulfur Cluster of Lipoyl Synthase from Mycobacterium Tuberculosis

      • Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, Squire J. Booker
      Pages 307-332
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Keywords

About this book

This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Editors and Affiliations

  • Department of Chemistry, Wake Forest University, Winston Salem, USA

    Patricia C. Dos Santos

Bibliographic Information

  • Book Title: Fe-S Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Patricia C. Dos Santos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1605-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1604-8Published: 23 July 2021

  • Softcover ISBN: 978-1-0716-1607-9Published: 24 July 2022

  • eBook ISBN: 978-1-0716-1605-5Published: 22 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 351

  • Number of Illustrations: 23 b/w illustrations, 64 illustrations in colour

  • Topics: Protein Science

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