Cytotoxic T-Cells

1. Front Matter

   Pages i-x

   PDF

2. Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting

   • Achille Anselmo, Federico Simone Colombo

   Pages 1-27

3. Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells

   • Margherita Gigante, Elena Ranieri

   Pages 29-39

4. Tumor Infiltrating T Cell Cytotoxicity Assay

   • Nada Chaoul, Marcello Albanesi

   Pages 41-54

5. Antigen-Specific In Vivo Killing Assay

   • Nada Chaoul, Gilles Dadaglio

   Pages 55-64

6. CTL ELISPOT Assay and T Cell Detection

   • Elena Ranieri, Giuseppe Stefano Netti, Margherita Gigante

   Pages 65-77

7. Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single-Cell RNA Sequencing

   • Rossana Franzin, Alessandra Stasi, Giuseppe Castellano, Loreto Gesualdo

   Pages 79-95

8. In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells

   • Giacomina Brunetti, Maria Grano

   Pages 97-106

9. Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray

   • Giuseppe Stefano Netti, Monica Rutigliano, Chiara Divella, Giuseppe Lucarelli

   Pages 107-124

10. A Review of Proteomics Strategies to Study T-Cell Activation and Function in Cancer Disease

    • Massimo Papale

    Pages 125-136

11. Methods to Study Posttranslational Modification Patterns in Cytotoxic T-Cells and Cancer

    • Annamaria Deleonardis, Massimo Papale

    Pages 137-153

12. Whole Exome Sequencing for the Identification of Mutations in CD8+ T-Cells

    • Sharon Natasha Cox

    Pages 155-182

13. Handling High-Throughput Omics Data for Systems Genetics Analysis

    • Francesco Pesce, Paolo Protopapa

    Pages 183-190

14. Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System

    • Francesca Conserva, Loreto Gesualdo, Paola Pontrelli

    Pages 191-202

15. Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview

    • Federica Rascio, Paola Pontrelli, Giuseppe Grandaliano

    Pages 203-213

16. Protein-Bound Uremic Toxins and Immunity

    • Maria Teresa Rocchetti, Carmela Cosola, Elena Ranieri, Loreto Gesualdo

    Pages 215-227

17. Gut Microbiota , the Immune System, and Cytotoxic T Lymphocytes

    • Carmela Cosola, Maria Teresa Rocchetti, Loreto Gesualdo

    Pages 229-241

18. Back Matter

    Pages 243-247

    PDF

This new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with in vitro and in vivo cytotoxicity assays, methods to detect senescent T cells, in vivo and in vitro models to understand immune and bone cells cross-talk, microscopy and in vivo imaging, as well as “Omics” approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and key implementation advice for best results in the lab. 

Authoritative and up-to-date, Cytotoxic T-Cells: Methods and Protocols, Second Edition serves as an ideal guidefor researchers working with these vital cells.

• T-cell responses
• CTL populations
• Systems biology
• Cytotoxicity assays
• Omics approaches

• Department of Surgical and Medical Sciences, University of Foggia, Foggia, Italy

  Margherita Gigante, Elena Ranieri

Policies and ethics