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Viroids

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Ayala L. N. Rao

    1. Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, USA

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  2. Irene Lavagi-Craddock

    1. Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Georgios Vidalakis

    1. Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2316)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Biological Techniques

    1. Front Matter

      Pages 1-1
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    2. Study and Detection of Citrus Viroids in Woody Hosts

      • Robert R. Krueger, Georgios Vidalakis
      Pages 3-21

    3. Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts

      • Rosemarie W. Hammond
      Pages 23-28

    4. Analysis on RNA Motif-Based RNA Trafficking in Plants

      • Heather N. Smith, Junfei Ma, Ying Wang
      Pages 29-37

    5. Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid

      • Subhas Hajeri, James Ng, Jude Grosser, Georgios Vidalakis
      Pages 39-54

  3. Electrophoresis and Hybridization Techniques

    1. Front Matter

      Pages 55-55
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    2. High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids

      • Tyler Dang, Fatima Osman, Jinbo Wang, Tavia Rucker, Sohrab Bodaghi, Shih-hua Tan et al.
      Pages 57-64

    3. Extraction and Purification of Viroids from Herbaceous Hosts

      • Rosemarie W. Hammond
      Pages 65-70

    4. Detection of Viroids by sPAGE Gel Electrophoresis

      • Núria Duran-Vila
      Pages 71-77

    5. Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Viroid RNAs

      • José-Antonio Daròs
      Pages 79-88

    6. A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae

      • Oxana Kekstidou, Christina Varveri, Nikon Vassilakos
      Pages 89-96

    7. Gel Blot Hybridization for Viroids

      • Pedro Serra
      Pages 97-109

    8. Tissue Print Hybridization for Detection and Characterization of Viroids

      • Maria S. Kaponi, Teruo Sano, Panayota E. Kyriakopoulou
      Pages 111-131

    9. Fluorescein-Based Electrophoretic Mobility Shift Assay

      • Shachinthaka D. Dissanayaka Mudiyanselage, Ying Wang
      Pages 133-140

  4. PCR Techniques

    1. Front Matter

      Pages 141-141
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    2. Detection of Viroids by RT-PCR

      • Nerida J. Donovan, Grant A. Chambers, Mengji Cao
      Pages 143-151

    3. Detection of Viroids Using RT-qPCR

      • Subhas Hajeri, Georgios Vidalakis, Raymond K. Yokomi
      Pages 153-162

    4. Multiplex RT-PCR

      • Francesco Faggioli, Marta Luigi
      Pages 163-179

    5. Real-Time Detection of Viroids Using Singleplex and Multiplex Quantitative Polymerase Chain Reaction

      • Fatima Osman, Georgios Vidalakis
      Pages 181-194
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Keywords

About this book

This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases. 


Editors and Affiliations

  • Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, USA

    Ayala L. N. Rao, Irene Lavagi-Craddock, Georgios Vidalakis

Bibliographic Information

  • Book Title: Viroids

  • Book Subtitle: Methods and Protocols

  • Editors: Ayala L. N. Rao, Irene Lavagi-Craddock, Georgios Vidalakis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1464-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1463-1Published: 30 November 2021

  • Softcover ISBN: 978-1-0716-1466-2Published: 30 November 2022

  • eBook ISBN: 978-1-0716-1464-8Published: 30 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 375

  • Number of Illustrations: 33 b/w illustrations, 48 illustrations in colour

  • Topics: Plant Genetics and Genomics

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