Mitochondrial Regulation

1. Front Matter

   Pages i-xii

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2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression

   • André L. Simão, Pedro M. Borralho, Clifford J. Steer, Rui E. Castro, Cecília M. P. Rodrigues

   Pages 1-15

3. Determination of Oxidative Phosphorylation Complexes Activities

   • João S. Teodoro, Ivo F. Machado, Carlos M. Palmeira, Anabela P. Rolo

   Pages 17-31

4. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

   • Elena Silvestri, Assunta Lombardi, Federica Cioffi, Fernando Goglia

   Pages 33-45

5. Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)

   • Bibiana Correia, Maria Inês Sousa, Ana F. Branco, João Ramalho-Santos

   Pages 47-56

6. Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes

   • Renata S. Tavares, Sara Escada-Rebelo, Maria M. Soares, Andreia F. Silva, Teresa Almeida-Santos, Sandra Amaral et al.

   Pages 57-68

7. Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections

   • Mariusz R. Wieckowski, Maciej Pronicki, Agnieszka Karkucinska-Wieckowska

   Pages 69-77

8. An Update on Isolation of Functional Mitochondria from Cells for Bioenergetics Studies

   • Magdalena Lebiedzinska-Arciszewska, Lech Wojtczak, Mariusz R. Wieckowski

   Pages 79-89

9. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species

   • Tess C. Leuthner, Jessica H. Hartman, Ian T. Ryde, Joel N. Meyer

   Pages 91-111

10. Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases

    • Simone Patergnani, Massimo Bonora, Esmaa Bouhamida, Alberto Danese, Saverio Marchi, Giampaolo Morciano et al.

    Pages 113-159

11. Analysis of Proapoptotic Protein Trafficking to and from Mitochondria

    • Ignacio Vega-Naredo, Gabriela Oliveira, Teresa Cunha-Oliveira, Teresa Serafim, Vilma A. Sardão, Paulo J. Oliveira

    Pages 161-178

12. Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

    • Emilio Molina-Molina, Harshitha Shanmugam, Domenica Di Palo, Ignazio Grattagliano, Piero Portincasa

    Pages 179-199

13. Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)

    • Ignazio Grattagliano, Agostino Di Ciaula, Jacek Baj, Emilio Molina-Molina, Harshitha Shanmugam, Gabriella Garruti et al.

    Pages 201-246

14. The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria

    • Andrea Dlasková, Kieran J. Clarke, Mary F. Rooney, Richard K. Porter

    Pages 247-258

15. Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis

    • Francisco X. Carvalho, Bárbara Guerra-Carvalho, Ivana Jarak, Rui A. Carvalho

    Pages 259-270

16. Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content

    • Yue Yang, Anthony A. Sauve

    Pages 271-285

17. Following the Dynamism of the Mitochondrial Network in T Cells

    • Arianna Di Daniele, Luca Simula, Silvia Campello

    Pages 287-299

18. Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes

    • João A. Amorim, David A. Sinclair

    Pages 301-309

19. Back Matter

    Pages 311-318

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This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.

• Mitochondria
• Dynamic properties
• Signaling events
• Cellular metabolism
• Mitochondrial function

• Department of Life Sciences, University of Coimbra, Coimbra, Portugal

  Carlos M. Palmeira, Anabela P. Rolo

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