Mass Spectrometry-Based Lipidomics

1. Front Matter

   Pages i-xi

   PDF

2. Mass Spectrometry-Based Lipidomics: An Overview

   • Fong-Fu Hsu

   Pages 1-10

3. High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry

   • Bailey S. Rose, Katrina L. Leaptrot, Rachel A. Harris, Stacy D. Sherrod, Jody C. May, John A. McLean

   Pages 11-37

4. Global Lipidomics Profiling by a High Resolution LC-MS Platform

   • Thomas Züllig, Martin Trötzmüller, Harald C. Köfeler

   Pages 39-51

5. Comprehensive Structural Characterization of Lipids by Coupling Paternò–Büchi Reaction and Tandem Mass Spectrometry

   • Qingyuan Hu, Yu Xia, Xiaoxiao Ma

   Pages 53-60

6. Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis

   • Vinzenz Hofferek, Huaqi Su, Gavin E. Reid

   Pages 61-75

7. Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics After Methylation

   • Meixia Pan, Chao Qin, Xianlin Han

   Pages 77-91

8. Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of Complex Long-Chain Fatty Acids

   • Cheryl Frankfater, Fong-Fu Hsu

   Pages 93-103

9. One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS

   • Charles H. Hocart, Abdeljalil El Habti, Gabriel O. James

   Pages 105-121

10. Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument

    • Fong-Fu Hsu

    Pages 123-137

11. Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High-Resolution Mass Spectrometry Platform

    • Meei-Hua Lin, Jeffrey H. Miner, Fong-Fu Hsu

    Pages 139-155

12. Mass Spectrometric Analysis of Meibomian Gland Lipids

    • Jianzhong Chen

    Pages 157-170

13. Quantification of Plasma Oxylipins Using Solid-Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry

    • Guan-yuan Chen, Qibin Zhang

    Pages 171-186

14. Targeted Lipidomics of Drosophila melanogaster During Development

    • Esther Xue Yi Goh, Xue Li Guan

    Pages 187-213

15. Shotgun Lipidomic Analysis of Leishmania Cells

    • Kai Zhang, Fong-Fu Hsu

    Pages 215-225

16. Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry

    • Raju V. V. Tatituri, Fong-Fu Hsu

    Pages 227-238

17. Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi

    • Ashutosh Singh, Maurizio Del Poeta

    Pages 239-255

18. Analytical Methodologies for Lipidomics in Hemp Plant

    • Andrea Cerrato, Anna Laura Capriotti, Carmela Maria Montone, Sara Elsa Aita, Giuseppe Cannazza, Cinzia Citti et al.

    Pages 257-273

19. Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry

    • Gérald Larrouy-Maumus

    Pages 275-283

20. Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-photoionization Mass Spectrometry

    • Chengyuan Liu, Fei Qi, Yang Pan

    Pages 285-298

This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves asan invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

• Shotgun lipidomics
• The lipidome
• LC-MS-based lipidomics
• MALDI-TOF
• Model organisms

• Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, USA

  Fong-Fu Hsu

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