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Doubled Haploid Technology

Volume 1: General Topics, Alliaceae, Cereals

  • Book
  • © 2021

Overview

Editors:
  1. Jose M. Segui-Simarro

    1. CPI, COMAV - Univ Politecnica Valencia, Valencia, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2287)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. General Topics and Transversal Methods in DH Technology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview of In Vitro and In Vivo Doubled Haploid Technologies

      • Jose M. Seguí-Simarro, Nathanaël M. A. Jacquier, Thomas Widiez
      Pages 3-22

    3. Applications of Doubled Haploids in Plant Breeding and Applied Research

      • Jens Weyen
      Pages 23-39

    4. Species with Haploid or Doubled Haploid Protocols

      • Jose M. Seguí-Simarro, Javier Belinchón Moreno, Marina Guillot Fernández, Ricardo Mir
      Pages 41-103

    5. Analysis of Ploidy in Haploids and Doubled Haploids

      • Sergio J. Ochatt, Jose M. Seguí-Simarro
      Pages 105-125

    6. Methods for Chromosome Doubling

      • Mehran E. Shariatpanahi, Mohsen Niazian, Behzad Ahmadi
      Pages 127-148

  3. Doubled Haploids in Alliaceae

    1. Front Matter

      Pages 149-149
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    2. Doubled Haploid Onion (Allium cepa L.) Production Via In Vitro Gynogenesis

      • Ali Ramazan Alan
      Pages 151-169

    3. In Vitro Gynogenesis in Leek (Allium ampeloprasum L.)

      • Fevziye Celebi-Toprak, Ali Ramazan Alan
      Pages 171-184

  4. Doubled Haploids in Cereals

    1. Front Matter

      Pages 185-185
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    2. Barley Isolated Microspore Culture

      • Luís Cistué, Begoña Echávarri
      Pages 187-197

    3. Site-Directed Mutagenesis in Barley Using RNA-Guided Cas Endonucleases During Microspore-Derived Generation of Doubled Haploids

      • Robert Eric Hoffie, Ingrid Otto, Hiroshi Hisano, Jochen Kumlehn
      Pages 199-214

    4. Generation of Doubled Haploid Barley by Interspecific Pollination with Hordeum bulbosum

      • Pooja Satpathy, Sara Audije de la Fuente, Vivian Ott, Andrea Müller, Heike Büchner, Diaa Eldin S. Daghma et al.
      Pages 215-226

    5. Bread Wheat Doubled Haploid Production by Anther Culture

      • Ana María Castillo, Isabel Valero-Rubira, Sandra Allué, María Asunción Costar, María Pilar Vallés
      Pages 227-244

    6. Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat

      • Olfa Ayed Slama, Hajer Slim Amara
      Pages 245-255

    7. In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat

      • Csaba Lantos, János Pauk
      Pages 257-266

    8. Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize

      • Pierre Devaux
      Pages 267-279

    9. Isolation of Staged and Viable Maize Microspores for DH Production

      • Philippe Vergne, Antoine Gaillard
      Pages 281-293

    10. Triticale Isolated Microspore Culture for Doubled Haploid Production

      • Priti Maheshwari, John D. Laurie
      Pages 295-312
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Keywords

About this book

This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 1 explores general topics and transversal methods in DH technology; DH production in two alliaceae, onion, and leek; and DH production in cereals such as barley, durum, bread, oat, and japonica rice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Doubled Haploid Technology, Volume 1: General Topics, Alliaceae, Cereals is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.



Editors and Affiliations

  • CPI, COMAV - Univ Politecnica Valencia, Valencia, Spain

    Jose M. Segui-Simarro

Bibliographic Information

  • Book Title: Doubled Haploid Technology

  • Book Subtitle: Volume 1: General Topics, Alliaceae, Cereals

  • Editors: Jose M. Segui-Simarro

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1315-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1314-6Published: 17 July 2021

  • Softcover ISBN: 978-1-0716-1317-7Published: 18 July 2022

  • eBook ISBN: 978-1-0716-1315-3Published: 16 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 363

  • Number of Illustrations: 3 b/w illustrations, 57 illustrations in colour

  • Topics: Biotechnology, Plant Genetics and Genomics

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