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T-Helper Cells

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Francesco Annunziato

    1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Laura Maggi

    1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

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    You can also search for this editor in PubMed   Google Scholar

  3. Alessio Mazzoni

    1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2285)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
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  2. Human and Murine T-Helper Cell Recovery from Organs and Tissues

    • Francesco Siracusa, Annette Lahmann, Anna Vanni, Filippo Cortesi, Alessio Mazzoni
    Pages 1-25

  3. Identification and Purification of Human Memory T Helper Cells from Peripheral Blood

    • Alessia Capone, Elisabetta Volpe
    Pages 27-34

  4. Transcription Factor Evaluation by Flow Cytometry

    • Daniela Fenoglio, Tiziana Altosole
    Pages 35-47

  5. Mass Cytometry Analysis of T-Helper Cells

    • Priyanka B. Subrahmanyam, Holden T. Maecker
    Pages 49-63

  6. Murine T-Helper Cell Differentiation and Plasticity

    • Francesco Siracusa, Franziska Muscate, Laura Garcia Perez
    Pages 65-75

  7. Identification of Follicular T-Cell Subsets in Murine and Human Tissues

    • Annette Lahmann, Laura Bauer, Andreas Hutloff
    Pages 77-90

  8. Analysis of T-Cells in Inflamed Nonlymphoid Tissues

    • Laura Bauer, Andreas Hutloff
    Pages 91-98

  9. TCR Vβ Evaluation by Flow Cytometry

    • Benedetta Peruzzi, Sara Bencini, Roberto Caporale
    Pages 99-109

  10. Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation

    • Jakob Zimmermann
    Pages 111-119

  11. Evaluation of Secreted Cytokines by Multiplex Bead-Based Assay (X MAP Technology, Luminex)

    • Letizia Lombardelli, Federica Logiodice, Ornela Kullolli, Marie-Pierre Piccinni
    Pages 121-130

  12. Quantifying Antigen-Specific T-Cells by Assessing Their Antigen-Induced Proliferation

    • Aayushi Shah, Hyun-Dong Chang
    Pages 131-139

  13. Flow Cytometric Characterization of Human Antigen-Reactive T-Helper Cells

    • Carina Saggau, Alexander Scheffold, Petra Bacher
    Pages 141-152

  14. Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies

    • I-Ting Chow, William W. Kwok
    Pages 153-163

  15. Human T-Cell Cloning by Limiting Dilution

    • Laura Maggi, Manuela Capone, Alessio Mazzoni
    Pages 165-172

  16. Human Germinal Center Reaction: Assessing T Follicular Helper-B Cells Interaction In Vitro

    • Emeline Thevenon, Vanessa Cornacchione, Elisabetta Traggiai, Francesca Rucci
    Pages 173-189

  17. Study of Signal Transduction Pathways by Phospho-Protein Evaluation

    • Manuela Capone, Alessio Mazzoni, Laura Maggi
    Pages 191-200

  18. The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation

    • Arnulf Hertweck, Richard G. Jenner
    Pages 201-216

  19. mRNA Transfection of T-Lymphocytes by Electroporation

    • Lea-Isabell Schwarze, Boris Fehse
    Pages 217-226

  20. Transduction of Human T Cell Subsets with Lentivirus

    • Vivian C. W. Fung, Isaac Rosado-Sánchez, Megan K. Levings
    Pages 227-254
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Keywords

About this book

The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.


Editors and Affiliations

  • Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Francesco Annunziato, Laura Maggi, Alessio Mazzoni

Bibliographic Information

  • Book Title: T-Helper Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Francesco Annunziato, Laura Maggi, Alessio Mazzoni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1311-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1310-8Published: 30 April 2021

  • Softcover ISBN: 978-1-0716-1313-9Published: 30 April 2022

  • eBook ISBN: 978-1-0716-1311-5Published: 30 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 388

  • Number of Illustrations: 52 b/w illustrations, 51 illustrations in colour

  • Topics: Immunology

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