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Helicobacter Pylori

  • Book
  • © 2021

Overview

Editors:
  1. Sinead M Smith

    1. Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2283)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Overview of Helicobacter pylori Infection

    • Rebecca FitzGerald, Sinéad M. Smith
    Pages 1-14

  3. The Urea Breath Test for the Noninvasive Detection of Helicobacter pylori

    • Anthony O’Connor
    Pages 15-20

  4. Real-Time Diagnosis of Helicobacter pylori During Endoscopy by Gastric Juice Analysis

    • Cristiano Spada, Stefania Piccirelli
    Pages 21-27

  5. Molecular Detection of Antibiotic-Resistant Helicobacter pylori

    • Denise Brennan, Colm O’Morain, Deirdre McNamara, Sinéad M. Smith
    Pages 29-36

  6. Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test

    • Vikrant Parihar, Deirdre McNamara
    Pages 37-43

  7. Culture-Based Antimicrobial Susceptibility Testing for Helicobacter pylori

    • Philippe Lehours, Francis Mégraud
    Pages 45-50

  8. Purification of Total RNA from Stomach Tissue Biopsies

    • Rebecca FitzGerald, Malini Devapal, John J. Hickey, Deirdre McNamara, Dermot Kelleher, Sinéad M. Smith
    Pages 51-59

  9. Gene Expression Analysis by Reverse Transcription Quantitative PCR

    • Eva M. Campion, Sinéad T. Loughran
    Pages 61-74

  10. A Method to Investigate the Helicobacter pylori-Associated DNA Methylome

    • Hae Dong Woo, Zdenko Herceg
    Pages 75-81

  11. Profiling the Gastrointestinal Microbiota

    • Brunella Posteraro, Flavio De Maio, Antonio Gasbarrini
    Pages 83-92

  12. Helicobacter pylori Virulence Factor Genotyping

    • Rebecca FitzGerald, Chavi Sinha, Abbas Yadegar, Sinéad M. Smith
    Pages 93-106

  13. Generation of Isogenic Mutant Strains of Helicobacter pylori

    • Stephanie L. Servetas, Jeannette M. Whitmire, D. Scott Merrell
    Pages 107-122

  14. Isolation of Outer Membrane Vesicles from Helicobacter pylori

    • Henry J. Windle
    Pages 123-130

  15. A Mouse Model of Helicobacter pylori Infection

    • Tanmoy Kumar Dey, Bipul Chandra Karmakar, Avijit Sarkar, Sangita Paul, Asish Kumar Mukhopadhyay
    Pages 131-151

  16. Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa

    • Marguerite Clyne, Ciara Dunne, Brendan Dolan
    Pages 153-173

  17. Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot

    • Ciara Nulty, Gillian Barber, Ewelina Flis, Emma M. Creagh
    Pages 175-190

  18. Analysis of Innate Immune Responses to Helicobacter pylori

    • Michelle Chonwerawong, Richard L. Ferrero
    Pages 191-214

  19. Analysis of T Cell Responses to Helicobacter pylori Infection

    • Kazuyo Kaneko, Karen Robinson
    Pages 215-223

  20. Miniaturized In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-Resistance

    • Niamh McNamee, Lorraine O’Driscoll
    Pages 225-232
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Keywords

About this book

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases.  Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Editors and Affiliations

  • Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland

    Sinead M Smith

Bibliographic Information

  • Book Title: Helicobacter Pylori

  • Editors: Sinead M Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1302-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1301-6Published: 26 March 2021

  • Softcover ISBN: 978-1-0716-1304-7Published: 25 March 2021

  • eBook ISBN: 978-1-0716-1302-3Published: 25 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 234

  • Number of Illustrations: 8 b/w illustrations, 30 illustrations in colour

  • Topics: Internal Medicine, Microbiology

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