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Lung Cancer

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Pedro G. Santiago-Cardona

    1. Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2279)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Automated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma

    • Rosa Vélez Cintrón, Andrés J. Martínez, Jo Ann Jusino, María Conte-Miller, Adalberto Mendoza
    Pages 1-12

  3. Immunohistochemical Detection of p40 Expression in Lung Cancer Clinical Samples

    • Aruna Nambirajan, Deepali Jain
    Pages 13-21

  4. Automated Immunohistochemistry Assay for the Detection of Napsin-A in Formalin-Fixed Paraffin-Embedded Tissues from Lung Tumors

    • Rosa Vélez Cintrón, Jo Ann Jusino, María Conte-Miller, Andrés J. Martínez, Adalberto Mendoza
    Pages 23-33

  5. Detection of Programmed Cell Death Ligand 1 Expression in Lung Cancer Clinical Samples by an Automated Immunohistochemistry System

    • Edwin Roger Parra, Sharia Hernández Ruiz
    Pages 35-47

  6. Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression

    • Edwin Roger Parra, Sharia Hernández Ruiz
    Pages 49-57

  7. Creation of Formalin-Fixed, Paraffin-Embedded 3D Lung Cancer Cellular Spheroids for the Optimization of Immunohistochemistry Staining Procedures

    • Jennifer Cabán-Rivera, Camille Chardón-Colón, Alberto Pedraza-Torres, Yoan E. Rodríguez, Raymond Quiñones-Alvarado, Pedro G. Santiago-Cardona
    Pages 59-73

  8. Immunoblot Validation of Phospho-Specific Antibodies Using Lung Cancer Cell Lines

    • Wilfredo M. Pedreira-García, Jaileene Pérez-Morales, Camille Chardón-Colón, Jennifer Cabán-Rivera, Pedro G. Santiago-Cardona
    Pages 75-90

  9. Detection of Non-Small Lung Cell Carcinoma-Associated Genetic Alterations Using a NanoString Gene Expression Platform Approach

    • Johan Staaf, Mats Jönsson, Anna F. Karlsson
    Pages 91-107

  10. A PCR-Based Approach for Driver Mutation Analysis of EGFR, KRAS, and BRAF Genes in Lung Cancer Tissue Sections

    • Rodrigo de Oliveira Cavagna, Leticia Ferro Leal, Flávia Escremim de Paula, Gustavo Noriz Bernardinelli, Rui Manuel Reis
    Pages 109-126

  11. 6-Color Crystal Digital PCRTM for the High-Plex Detection of EGFR Mutations in Non-Small Cell Lung Cancer

    • Jordan Madic, Cécile Jovelet, Imane Dehri, Allison C. Mallory
    Pages 127-144

  12. Detection of MET Exon 14 Skipping Alterations in Lung Cancer Clinical Samples Using a PCR-Based Approach

    • Jane S. Y. Sui, Stephen P. Finn, Steven G. Gray
    Pages 145-155

  13. Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

    • Diane Frankel, Elise Kaspi, Patrice Roll
    Pages 157-164

  14. A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice

    • Joanne Lundy, Brendan J. Jenkins, Mohamed I. Saad
    Pages 165-173

  15. A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas

    • Magda Spella, Ioannis Lilis, Georgios T. Stathopoulos
    Pages 175-186

  16. Whole Transcriptome Sequencing Analysis of Cancer Stem/Progenitor Cells Obtained from Mouse Lung Adenocarcinomas

    • Ansam Sinjab, Reem Daouk, Wassim Abou-Kheir, Humam Kadara
    Pages 187-198

  17. In Vivo Imaging of Orthotopic Lung Cancer Models in Mice

    • Peng Liu, Liwei Zhao, Laura Senovilla, Oliver Kepp, Guido Kroemer
    Pages 199-212

  18. An Annexin V-FITC—Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line

    • Robin Kumar, Ankit Saneja, Amulya K. Panda
    Pages 213-223

  19. Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach

    • Angélica B. Sanchez, Camila C. M. Garcia, Paolo Di Mascio, Marisa H. G. Medeiros
    Pages 225-239

  20. A Method for Liposome Co-encapsulation of Phenethyl Isothiocyanate and Cisplatin and Determining Its Toxicity Against Lung and Lung Cancer Cells

    • Mengwei Sun, Anthony J. Di Pasqua
    Pages 241-253
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Keywords

About this book

This detailed book serves as a laboratory manual containing vital protocols and in-depth discussion involving commonly used experimental approaches for the characterization of several aspects of lung tumor biology. Beginning with an extensive section on biomarker detection, the volume continues with chapters on the genetic and molecular characterization of lung cancer biological samples as well as protocols for the generation of research tools and pre-clinical lung cancer models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Lung Cancer: Methods and Protocols provides a global perspective of research efforts related to lung cancer, while allowing researchers to experimentally probe the different aspects of lung cancer research, including the experimentally relevant tests used in the establishment of lung cancer diagnosis and prognosis, in their laboratories.

Editors and Affiliations

  • Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico

    Pedro G. Santiago-Cardona

Bibliographic Information

  • Book Title: Lung Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Pedro G. Santiago-Cardona

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1278-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1277-4Published: 09 March 2021

  • Softcover ISBN: 978-1-0716-1280-4Published: 09 March 2022

  • eBook ISBN: 978-1-0716-1278-1Published: 08 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 258

  • Number of Illustrations: 2 b/w illustrations, 43 illustrations in colour

  • Topics: Cancer Research

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