Skip to main content
SpringerLink
Log in

Live Cell Imaging

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Sung-Bae Kim

    1. National Institute of Advanced Industrial Science and Technology, Research Institute for Environmental Management Technology, Tsukuba, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2274)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Imaging with Passive Optical Readouts

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins

      • Toshiyuki Taniyama, Shinji Sueda
      Pages 3-14

    3. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools

      • Osamu Takenouchi, Hideaki Yoshimura, Takeaki Ozawa
      Pages 15-23

    4. Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging

      • Bryce T. Bajar, Michael Z. Lin
      Pages 25-35

    5. A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging

      • Takahiro Kuchimaru, Shinae Kizaka-Kondoh
      Pages 37-42

    6. A New Lineage of Artificial Luciferases for Mammalian Cell Imaging

      • Sung-Bae Kim, Rika Fujii
      Pages 43-51

    7. Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems

      • Jittima Phonbuppha, Ruchanok Tinikul, Pimchai Chaiyen
      Pages 53-65

  3. Imaging with Activatable Bioluminescent Probes

    1. Front Matter

      Pages 67-67
      Download chapter PDF

    2. Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation

      • Timo Littmann, Takeaki Ozawa, Günther Bernhardt
      Pages 69-78

    3. A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules

      • Qiaojing Li, Hideaki Yoshimura, Takeaki Ozawa
      Pages 79-87

    4. Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution

      • Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi
      Pages 89-100

  4. Imaging with Functional Substrates and Luciferases

    1. Front Matter

      Pages 101-101
      Download chapter PDF

    2. Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette

      • Masahiro Abe, Ryo Nishihara, Sung-Bae Kim, Koji Suzuki
      Pages 103-110

    3. Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells

      • Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Koji Suzuki, Sung-Bae Kim
      Pages 111-126

    4. Luciferase-Specific Coelenterazine Analogues for Optical Cross Talk-Free Bioassays

      • Ryo Nishihara, Masahiro Abe, Koji Suzuki, Sung-Bae Kim
      Pages 127-138

  5. Imaging with Organic Fluorescent Probes

    1. Front Matter

      Pages 139-139
      Download chapter PDF

    2. Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe

      • Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, Takashi Suzuki
      Pages 141-154

    3. Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe

      • Mitsuyasu Kawaguchi, Hidehiko Nakagawa
      Pages 155-168

    4. Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Derived Peptide

      • Hiroshi Inaba, Kazunori Matsuura
      Pages 169-179
Back to top

Keywords

About this book

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Editors and Affiliations

  • National Institute of Advanced Industrial Science and Technology, Research Institute for Environmental Management Technology, Tsukuba, Japan

    Sung-Bae Kim

Bibliographic Information

  • Book Title: Live Cell Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Sung-Bae Kim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1258-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1257-6Published: 29 May 2021

  • Softcover ISBN: 978-1-0716-1260-6Published: 30 May 2022

  • eBook ISBN: 978-1-0716-1258-3Published: 28 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 447

  • Number of Illustrations: 29 b/w illustrations, 109 illustrations in colour

  • Topics: Cell Biology, Analytical Chemistry, Biological Techniques

Publish with us

Policies and ethics

Back to top

Search

Navigation