Methods in Molecular Biology

Mass Spectrometry of Glycoproteins

Methods and Protocols

Editors: Delobel, Arnaud (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 109,99 €
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  • ISBN 978-1-0716-1241-5
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Hardcover 145,59 €
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  • ISBN 978-1-0716-1240-8
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About this book

This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Glycosylation of Therapeutic Proteins: A Critical Quality Attribute

    Pages 1-21

    Delobel, Arnaud

  • Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry

    Pages 23-45

    Kristensen, Dan Bach (et al.)

  • Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

    Pages 47-56

    Giménez, Estela (et al.)

  • Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS

    Pages 57-71

    Helali, Yosra (et al.)

  • Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry

    Pages 73-83

    Wagner-Rousset, Elsa (et al.)

Buy this book

eBook 109,99 €
price for Spain (gross)
  • ISBN 978-1-0716-1241-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-0716-1240-8
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Mass Spectrometry of Glycoproteins
Book Subtitle
Methods and Protocols
Editors
  • Arnaud Delobel
Series Title
Methods in Molecular Biology
Series Volume
2271
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1241-5
DOI
10.1007/978-1-0716-1241-5
Hardcover ISBN
978-1-0716-1240-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 401
Number of Illustrations
14 b/w illustrations, 126 illustrations in colour
Topics