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Methods in Molecular Biology

G Protein-Coupled Receptor Screening Assays

Methods and Protocols

Editors: Martins, Sofia Aires M., Prazeres, Duarte Miguel F. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook 139,09 €
price for Spain (gross)
  • ISBN 978-1-0716-1221-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-0716-1220-0
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy

    Pages 1-20

    Abdelmaseeh, Peter (et al.)

  • Detection of GPCR mRNA Expression in Primary Cells Via qPCR, Microarrays, and RNA-Sequencing

    Pages 21-42

    Sriram, Krishna (et al.)

  • Construction of Recombinant Cell Lines for GPCR Expression

    Pages 43-60

    Reeves, Philip J.

  • Recombinant Expression and Purification of Cannabinoid Receptor CB

    Pages 61-76

    Yeliseev, Alexei A.

  • Screening for Serotonin Receptor 4 Agonists Using a GPCR-Based Sensor in Yeast

    Pages 77-84

    Yasi, Emily A. (et al.)

Buy this book

eBook 139,09 €
price for Spain (gross)
  • ISBN 978-1-0716-1221-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-0716-1220-0
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
G Protein-Coupled Receptor Screening Assays
Book Subtitle
Methods and Protocols
Editors
  • Sofia Aires M. Martins
  • Duarte Miguel F. Prazeres
Series Title
Methods in Molecular Biology
Series Volume
2268
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1221-7
DOI
10.1007/978-1-0716-1221-7
Hardcover ISBN
978-1-0716-1220-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 327
Number of Illustrations
9 b/w illustrations, 69 illustrations in colour
Topics