- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
-
This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer.
Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Table of contents (45 chapters)
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Model Systems for the Study of Malignant Melanoma
Pages 1-21
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Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing
Pages 25-46
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A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix
Pages 47-63
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Wound Healing Assay for Melanoma Cell Migration
Pages 65-71
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A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells
Pages 73-80
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Table of contents (45 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Melanoma
- Book Subtitle
- Methods and Protocols
- Editors
-
- Kristian M. Hargadon
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2265
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1205-7
- DOI
- 10.1007/978-1-0716-1205-7
- Hardcover ISBN
- 978-1-0716-1204-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XX, 674
- Number of Illustrations
- 25 b/w illustrations, 97 illustrations in colour
- Topics
