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Melanoma

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kristian M. Hargadon

    1. Department of Biology, Hampden-Sydney College, Hampden-Sydney, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2265)

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Table of contents (45 protocols)

  1. Front Matter

    Pages i-xx
    Download chapter PDF

  2. Model Systems for the Study of Malignant Melanoma

    • Randal K. Gregg
    Pages 1-21

  3. 2D Cell Culture-Based Approaches for Studying Melanoma Biology

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing

      • Kristian M. Hargadon, David Z. Bushhouse, Coleman E. Johnson, Corey J. Williams
      Pages 25-46

    3. A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix

      • Ewa Mazurkiewicz, Ewa MrówczyÅ„ska, Aleksandra Simiczyjew, Dorota Nowak, Antonina J. Mazur
      Pages 47-63

    4. Wound Healing Assay for Melanoma Cell Migration

      • Juliano T. Freitas, Ivan Jozic, Barbara Bedogni
      Pages 65-71

    5. A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells

      • Jelena Grahovac, Marijana Pavlović, Marija Ostojić
      Pages 73-80

    6. Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology

      • Ashley V. Menk, Greg M. Delgoffe
      Pages 81-89

    7. Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry

      • David A. Scott
      Pages 91-110

    8. Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry

      • Guilan Shi, Richard Heller
      Pages 111-118

    9. In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium

      • Tao Wang, Russel E. Kaufman
      Pages 119-128

    10. A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells

      • Kristian M. Hargadon, Coleman E. Johnson
      Pages 129-138

  4. 3D Cell Culture Systems for Studying Melanoma

    1. Front Matter

      Pages 139-139
      Download chapter PDF

    2. An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence

      • Sanni Alve, Silvia Gramolelli, Päivi M. Ojala
      Pages 141-154

    3. Evaluating Melanoma Viability and Proliferation in 3D Microenvironments

      • Vasanth Siruvallur Murali, Murat Can Cobanoglu, Erik S. Welf
      Pages 155-171

    4. Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models

      • Maximilian E. A. Schäfer, Julia Klicks, Mathias Hafner, Rüdiger Rudolf
      Pages 173-183

    5. Enrichment of Melanoma Stem-Like Cells via Sphere Assays

      • Nabanita Mukherjee, Karoline A. Lambert, David A. Norris, Yiqun G. Shellman
      Pages 185-199Open Access

  5. Techniques for Isolating and Studying Circulating Melanoma Cells

    1. Front Matter

      Pages 201-201
      Download chapter PDF

    2. Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry

      • Robert H. Edgar, Justin Cook, Madeline Douglas, Anie-Pier Samson, John A. Viator
      Pages 203-212

    3. Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

      • Aaron B. Beasley, Emmanuel Acheampong, Weitao Lin, Elin S. Gray
      Pages 213-222
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Keywords

About this book

This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer.



Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Biology, Hampden-Sydney College, Hampden-Sydney, USA

    Kristian M. Hargadon

Bibliographic Information

  • Book Title: Melanoma

  • Book Subtitle: Methods and Protocols

  • Editors: Kristian M. Hargadon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1205-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1204-0Published: 12 March 2021

  • Softcover ISBN: 978-1-0716-1207-1Published: 13 March 2022

  • eBook ISBN: 978-1-0716-1205-7Published: 11 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 674

  • Number of Illustrations: 25 b/w illustrations, 97 illustrations in colour

  • Topics: Cancer Research, Biomedicine, general

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