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Host-Fungal Interactions

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2260)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. CRISPR-Cas9-Mediated Gene Silencing in Cultured Human Epithelia

    • Sara Gago, Nicola L. D. Overton, Paul Bowyer
    Pages 37-47
  3. Analysis of Epithelial Cell Responses to Microbial Pathogens

    • Spyridoula Nikou, Jemima Ho, Olivia Hepworth, Nicole Ponde, Ruth Dickenson, Jonathan P. Richardson et al.
    Pages 49-82
  4. Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

    • Jeffrey Scott Fites, Neta Shlezinger, Tobias M. Hohl, Bruce S. Klein
    Pages 111-119
  5. Fungal Bioreporters to Monitor Outcomes of Aspergillus: Host–Cell Interactions

    • Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, Tobias M. Hohl
    Pages 121-132
  6. Experimental Evolution of Candida by Serial Passaging in Host Cells

    • Katja Graf, Bernhard Hube, Sascha Brunke
    Pages 145-154
  7. Quantifying Receptor-Mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells

    • Patawee Asamaphan, Gordon D. Brown, Janet A. Willment
    Pages 155-178
  8. A Murine Model for Chronic A. fumigatus Airway Infections

    • Benjamin Ralph, Donald C. Sheppard
    Pages 215-224
  9. Automated Quantitative Analysis of Airway Epithelial Cell Detachment Upon Fungal Challenge

    • Sayema Rahman, Darren D. Thomson, Margherita Bertuzzi
    Pages 225-239
  10. Back Matter

    Pages 241-242

About this book

This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • MRC Centre for Medical Mycology, University of Exeter, Exeter, UK

    Elaine Bignell

Bibliographic Information

  • Book Title: Host-Fungal Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Elaine Bignell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1182-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1181-4Published: 07 January 2021

  • Softcover ISBN: 978-1-0716-1184-5Published: 07 January 2022

  • eBook ISBN: 978-1-0716-1182-1Published: 06 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 242

  • Number of Illustrations: 11 b/w illustrations, 28 illustrations in colour

  • Topics: Microbiology, Mycology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access