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Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

Methods and Concepts

  • Book
  • © 2021

Overview

Editors:
  1. Nuno F. Azevedo

    1. LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Porto, Portugal

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  2. Carina Almeida

    1. INIAV – National Institute for Agrarian and Veterinarian Research, Rua dos Lagidos, Lugar da Madalena, Vairão, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Vila do Conde, Portugal

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2246)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. An Introduction to Fluorescence in situ Hybridization in Microorganisms

    • Carina Almeida, Nuno F. Azevedo
    Pages 1-15

  3. FISH Variants

    • Nuno M. Guimarães, Nuno F. Azevedo, Carina Almeida
    Pages 17-33

  4. Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes

    • Helena Teixeira, Ana L. Sousa, Andreia S. Azevedo
    Pages 35-50

  5. FISH in Suspension or in Adherent Cells

    • Francesca Di Pippo, Diogo Queirós, Joana Pereira, Paulo C. Lemos, Luísa S. Serafim, Simona Rossetti
    Pages 51-67

  6. Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization

    • Ricardo Oliveira, Andreia S. Azevedo, Luzia Mendes
    Pages 69-86

  7. Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes

    • Sara Pereira, Rita S. Santos, Luis Moreira, Nuno M. Guimarães, Kevin Braeckmans, Stefaan C. De Smedt et al.
    Pages 87-96

  8. Characterization of Social Interactions and Spatial Arrangement of Individual Bacteria in MultiStrain or Multispecies Biofilm Systems Using Nucleic Acid Mimics-Fluorescence In Situ Hybridization

    • Jontana Allkja, Andreia S. Azevedo
    Pages 97-109

  9. Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces

    • Elena L. Peredo, Sheri Simmons
    Pages 111-128

  10. CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples

    • Bruna Matturro, Simona Rossetti, Patrícia Leitão
    Pages 129-140

  11. Fluorescence In Situ Hybridization with Quantum Dot Labels in E. coli Cells

    • Yang Liu, Zhiyuan Han, Suresh Sarkar, Andrew M. Smith
    Pages 141-155

  12. Monitoring Bacteriophage Infection on Bacterial Cells Using FISH

    • Diana Vilas-Boas, Luís D. R. Melo
    Pages 157-168

  13. Linking Microbes to Their Genes at Single Cell Level with Direct-geneFISH

    • Jimena Barrero-Canosa, Cristina Moraru
    Pages 169-205

  14. Assigning Function to Phylogeny: FISH-nanoSIMS

    • Katharina Kitzinger, Daniela Tienken, Sten Littmann, Abiel T. Kidane, Marcel M. M. Kuypers, Jana Milucka
    Pages 207-224

  15. Assigning Function to Phylogeny: MAR-FISH

    • Jeppe L. Nielsen
    Pages 225-236

  16. Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-Based Fluorescence In Situ Hybridization (fliFISH)

    • Dehong Hu, Yi Cui, Lye M. Markillie, William B. Chrisler, Qian Wang, Roland Hatzenpichler et al.
    Pages 237-247

  17. Integration of FISH and Microfluidics

    • Célia F. Rodrigues, Nuno F. Azevedo, João M. Miranda
    Pages 249-261

  18. Flow-FISH Using Nucleic Acid Mimic Probes for the Detection of Bacteria

    • Andreia S. Azevedo, Rui Rocha, Nicolina Dias
    Pages 263-277

  19. FISH in Food Samples

    • Rui Rocha, Carina Almeida, Nuno F. Azevedo
    Pages 279-290

  20. Extraction of Microbial Cells from Environmental Samples for FISH Approaches

    • Jennifer Pratscher
    Pages 291-299
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Keywords

About this book

This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge,  Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Porto, Portugal

    Nuno F. Azevedo

  • INIAV – National Institute for Agrarian and Veterinarian Research, Rua dos Lagidos, Lugar da Madalena, Vairão, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Vila do Conde, Portugal

    Carina Almeida

Bibliographic Information

  • Book Title: Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

  • Book Subtitle: Methods and Concepts

  • Editors: Nuno F. Azevedo, Carina Almeida

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1115-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1114-2Published: 13 February 2021

  • Softcover ISBN: 978-1-0716-1117-3Published: 13 February 2022

  • eBook ISBN: 978-1-0716-1115-9Published: 12 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 334

  • Number of Illustrations: 2 b/w illustrations, 50 illustrations in colour

  • Topics: Cell Biology, Microbiology

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