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Molecular Plant Taxonomy

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Pascale Besse

    1. UMR PVBMT, Universite de la Reunion, St Pierre, France

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2222)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives

    • Germinal Rouhan, Myriam Gaudeul
    Pages 1-38

  3. Guidelines for the Choice of Sequences for Molecular Plant Taxonomy

    • Pascale Besse
    Pages 39-55

  4. Isolation and Purification of DNA from Complicated Biological Samples

    • Ruslan Kalendar, Svetlana Boronnikova, Mervi Seppänen
    Pages 57-67

  5. Herbarium Specimens: A Treasure for DNA Extraction, an Update

    • Lenka Záveská Drábková
    Pages 69-88

  6. Sequencing of Complete Chloroplast Genomes

    • Berthold Heinze
    Pages 89-105

  7. Utility of the Mitochondrial Genome in Plant Taxonomic Studies

    • Jérôme Duminil, Guillaume Besnard
    Pages 107-118

  8. Nuclear Ribosomal RNA Genes: ITS Region

    • Pascale Besse
    Pages 119-129

  9. Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla

    • Pascale Besse, Denis Da Silva, Michel Grisoni
    Pages 131-148

  10. High-Throughput Genotyping Technologies in Plant Taxonomy

    • Monica F. Danilevicz, Cassandria G. Tay Fernandez, Jacob I. Marsh, Philipp E. Bayer, David Edwards
    Pages 149-166

  11. Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny

    • Félicien Favre, Cyril Jourda, Pascale Besse, Carine Charron
    Pages 167-178

  12. Development of Microsatellite Markers Using Next-Generation Sequencing

    • Hélène Vignes, Ronan Rivallan
    Pages 179-186

  13. Amplified Fragment Length Polymorphism: Applications and Recent Developments

    • Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, Kantipudi Nirmal Babu
    Pages 187-218

  14. Random Amplified Polymorphic DNA (RAPD) and Derived Techniques

    • Kantipudi Nirmal Babu, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby et al.
    Pages 219-247

  15. Inter-Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers

    • Chrissen E. C. Gemmill, Ella R. P. Grierson
    Pages 249-262

  16. Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity

    • Ruslan Kalendar, Alexander Muterko, Svetlana Boronnikova
    Pages 263-286

  17. Introduction to Population Genomics Methods

    • Thibault Leroy, Quentin Rougemont
    Pages 287-324

  18. The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants

    • Jaume Pellicer, Robyn F. Powell, Ilia J. Leitch
    Pages 325-361

  19. Molecular Cytogenetics (Fluorescence In Situ Hybridization - FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization

    • Sonja Siljak-Yakovlev, Fatima Pustahija, Vedrana Vičić-Bočkor, Odile Robin
    Pages 363-379

  20. GISH: Resolving Interspecific and Intergeneric Hybrids

    • Nathalie Piperidis
    Pages 381-394
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Keywords

About this book

This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an “integrative taxonomy” framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date,Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.

Editors and Affiliations

  • UMR PVBMT, Universite de la Reunion, St Pierre, France

    Pascale Besse

Bibliographic Information

  • Book Title: Molecular Plant Taxonomy

  • Book Subtitle: Methods and Protocols

  • Editors: Pascale Besse

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0997-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0996-5Published: 11 December 2020

  • Softcover ISBN: 978-1-0716-0999-6Published: 12 December 2021

  • eBook ISBN: 978-1-0716-0997-2Published: 10 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 400

  • Number of Illustrations: 29 b/w illustrations, 49 illustrations in colour

  • Topics: Plant Sciences, Animal Systematics/Taxonomy/Biogeography

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