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Osteoporosis and Osteoarthritis

  • Book
  • © 2021

Overview

Editors:
  1. Andre J. van Wijnen

    1. Stem Cell Therapy and Skeletal Regeneration Lab, Mayo Clinic, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Marina S. Ganshina

    1. Stem Cell Therapy and Skeletal Regeneration Lab, Mayo Clinic, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2221)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cellular and Molecular Biology of Osteoarthritis and Osteoporosis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation of Murine and Human Osteocytes

      • Matthew Prideaux, Amber Rath Stern, Lynda F. Bonewald
      Pages 3-13

    3. Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

      • Roberto Narcisi, Wendy J. L. M. Koevoet, Gergo J. V. M. van Osch
      Pages 15-28

    4. A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors

      • Leilei Zhong, Lutian Yao, Ling Qin
      Pages 29-39

    5. Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

      • Guus G. H. van den Akker, Andy Cremers, Donatus A. M. Surtel, Willem Voncken, Tim J. M. Welting
      Pages 41-52

    6. Engineering Cartilage Tissue by Co-culturing of Chondrocytes and Mesenchymal Stromal Cells

      • Yao Fu, Carlo A. Paggi, Amel Dudakovic, Andre J. van Wijnen, Janine N. Post, Marcel Karperien
      Pages 53-70

    7. Generation of Induced Pluripotent Stem Cells

      • David R. Deyle
      Pages 71-87

    8. Specimen Preparation for Single-Cell Sequencing Analysis of Skeletal Cells

      • Shawon Debnath, Matthew B. Greenblatt
      Pages 89-100

    9. Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing

      • Fiorella Carla Grandi, Nidhi Bhutani
      Pages 101-108

    10. Using FRAP to Quantify Changes in Transcription Factor Dynamics After Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization

      • Kannan Govindaraj, Janine N. Post
      Pages 109-139

    11. Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO

      • Sakshi Khurana, Janet Huisman, Stefano Schivo, Janine N. Post
      Pages 141-161

  3. In Vivo Models of Skeletal Tissue Injury, Degeneration, and Repair

    1. Front Matter

      Pages 163-163
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    2. Generation and Characterization of Mouse Models for Skeletal Disease

      • Gabrielle E. Foxa, Ye Liu, Lisa M. Turner, Alexander G. Robling, Tao Yang, Bart O. Williams
      Pages 165-191

    3. Drill Hole Models to Investigate Bone Repair

      • Zhijun Li, Jill A. Helms
      Pages 193-204

    4. Generation and Experimental Outcomes of Closed Femoral Fracture in Mice

      • Joseph L. Roberts, Christopher W. Kinter, Hicham Drissi
      Pages 205-222

    5. Mouse Models of Osteoarthritis: Surgical Model of Post-traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus

      • Kirsty L. Culley, Purva Singh, Samantha Lessard, Mengying Wang, Brennan Rourke, Mary B. Goldring et al.
      Pages 223-260

    6. Immunostaining of Skeletal Tissues

      • Crystal Idleburg, Madelyn R. Lorenz, Elizabeth N. DeLassus, Erica L. Scheller, Deborah J. Veis
      Pages 261-273

    7. Mapping Regional Cortical Bone Responses to Local Changes in Loading and Systemic Stimuli

      • Sara H. Windahl, Peter J. Delisser, Gabriel L. Galea
      Pages 275-289

    8. Pain and Activity Measurements

      • David H. H. Molstad, Elizabeth W. Bradley
      Pages 291-299
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Keywords

About this book

This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration. The first half of the book examines cell and molecular biological aspects of osteoarthritis and osteoporosis, while the remainder of the collection focuses on in vivo models of skeletal tissue injury, degeneration, and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Osteoporosis and Osteoarthritis, Second Edition aims to assist investigators concerned with research topics broadly related to osteoporosis, osteoarthritis, intervertebral disc degeneration, as well as other musculoskeletal disorders.

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Editors and Affiliations

  • Stem Cell Therapy and Skeletal Regeneration Lab, Mayo Clinic, Rochester, USA

    Andre J. van Wijnen, Marina S. Ganshina

Bibliographic Information

  • Book Title: Osteoporosis and Osteoarthritis

  • Editors: Andre J. van Wijnen, Marina S. Ganshina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0989-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0988-0Published: 27 September 2020

  • Softcover ISBN: 978-1-0716-0991-0Published: 27 September 2021

  • eBook ISBN: 978-1-0716-0989-7Published: 26 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 302

  • Number of Illustrations: 4 b/w illustrations, 59 illustrations in colour

  • Topics: Molecular Medicine, Orthopedics

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