- Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
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This volume provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
- Table of contents (26 chapters)
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Key Points to Consider When Studying RNA Remodeling by Proteins
Pages 1-16
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Happy Birthday: 30 Years of RNA Helicases
Pages 17-34
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Known Inhibitors of RNA Helicases and Their Therapeutic Potential
Pages 35-52
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Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays
Pages 53-72
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Thermal Shift Assay for Characterizing the Stability of RNA Helicases and Their Interaction with Ligands
Pages 73-85
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- RNA Remodeling Proteins
- Book Subtitle
- Methods and Protocols
- Editors
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- Marc Boudvillain
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2209
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0935-4
- DOI
- 10.1007/978-1-0716-0935-4
- Hardcover ISBN
- 978-1-0716-0934-7
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIV, 437
- Number of Illustrations
- 20 b/w illustrations, 57 illustrations in colour
- Topics
