Polypeptide Materials

1. Front Matter

   Pages i-xiv

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2. A Shortcut to the Synthesis of Peptide Thioesters

   • Richard Raz, John Offer

   Pages 1-12

3. SnoopLigase-Mediated Peptide–Peptide Conjugation and Purification

   • Can M. Buldun, Irsyad N. A. Khairil Anuar, Mark Howarth

   Pages 13-31

4. Peptide Nanoparticles for Gene Packaging and Intracellular Delivery

   • Paula Vila-Gómez, James E. Noble, Maxim G. Ryadnov

   Pages 33-48

5. Synthesis and Application of Peptide–siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung

   • Jade J. Welch, David A. Dean, Bradley L. Nilsson

   Pages 49-67

6. FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

   • Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, Jonathan G. Heddle

   Pages 69-80

7. Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes

   • Irene Marzuoli, Franca Fraternali

   Pages 81-100

8. Functional Peptide Nanocapsules Self-Assembled from β-Annulus Peptides

   • Hiroshi Inaba, Kazunori Matsuura

   Pages 101-121

9. Electrostatic Self-Assembly of Protein Cage Arrays

   • Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, Mauri A. Kostiainen

   Pages 123-133

10. Design and Generation of Self-Assembling Peptide Virus-like Particles with Intrinsic GPCR Inhibitory Activity

    • Sergey G. Tarasov, Marzena Dyba, Joshua Yu, Nadya Tarasova

    Pages 135-148

11. Imaging and 3D Reconstruction of De Novo Peptide Capsids

    • Emiliana De Santis, Maxim G. Ryadnov

    Pages 149-165

12. Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra

    • Abeer Alghamdi, Li Hung C. Chung, Olaf J. Rolinski

    Pages 167-177

13. Forming Low-Molecular-Weight Hydrogels by Electrochemical Methods

    • Emily R. Cross, Kate McAulay, Dave J. Adams

    Pages 179-188

14. In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study

    • Tristan O. C. Kwan, Rosana Reis, Isabel Moraes

    Pages 189-202

15. Measurement of Peptide Coating Thickness and Chemical Composition Using XPS

    • David J. H. Cant, Alexander G. Shard, Caterina Minelli

    Pages 203-224

16. Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy

    • Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom et al.

    Pages 225-235

17. Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials

    • Jehangir Cama, Stefano Pagliara

    Pages 237-253

18. Ultramicrotomy Analysis of Peptide-Treated Cells

    • Stephanie Rey, Nilofar Faruqui, Maxim G. Ryadnov

    Pages 255-264

19. Back Matter

    Pages 265-268

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This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

• Polypeptide materials
• Peptide thioesters
• Nanoscale
• Gene packaging systems
• Protein shell architecture
• Virus-like particles
• Microscopy

• National Physical Laboratory, Teddington, UK

  Maxim G. Ryadnov

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