Mitochondrial Gene Expression
Methods and Protocols
Editors: Minczuk, Michal, Rorbach, Joanna (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume details the most recent advancements in the field of mitochondrial gene expression. Chapters guide readers through methods and protocols on mtDNA replication, transcription, and translation to membrane insertion of the mtDNA-encoded protein products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines.Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Table of contents (21 chapters)
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In Vitro Analysis of mtDNA Replication
Pages 1-20
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In Vivo Analysis of mtDNA Replication at the Single Molecule Level and with High Resolution
Pages 21-34
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In Vitro Reconstitution of Human Mitochondrial Transcription
Pages 35-41
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Investigating Mitochondrial Transcriptomes and RNA Processing Using Circular RNA Sequencing
Pages 43-57
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Detection of 5-formylcytosine in Mitochondrial Transcriptome
Pages 59-68
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Table of contents (21 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Mitochondrial Gene Expression
- Book Subtitle
- Methods and Protocols
- Editors
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- Michal Minczuk
- Joanna Rorbach
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2192
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0834-0
- DOI
- 10.1007/978-1-0716-0834-0
- Hardcover ISBN
- 978-1-0716-0833-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 333
- Number of Illustrations
- 19 b/w illustrations, 40 illustrations in colour
- Topics
