- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.
- Table of contents (16 chapters)
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Preparation of Fragaceatoxin C (FraC) Nanopores
Pages 3-10
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Preparation of Cytolysin A (ClyA) Nanopores
Pages 11-18
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Building Synthetic Transmembrane Peptide Pores
Pages 19-32
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Design and Assembly of Membrane-Spanning DNA Nanopores
Pages 33-48
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Determining the Orientation of Porins in Planar Lipid Bilayers
Pages 51-62
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Nanopore Technology
- Book Subtitle
- Methods and Protocols
- Editors
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- Monifa A.V. Fahie
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2186
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0806-7
- DOI
- 10.1007/978-1-0716-0806-7
- Hardcover ISBN
- 978-1-0716-0805-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 231
- Number of Illustrations
- 14 b/w illustrations, 43 illustrations in colour
- Topics
