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Protein Downstream Processing

Design, Development, and Application of High and Low-Resolution Methods

  • Book
  • © 2021

Overview

Editors:
  1. Nikolaos E. Labrou

    1. Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2178)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Screening and Design Purification Strategies

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Protein Purification Technologies

      • Nikolaos E. Labrou
      Pages 3-10

    3. High-Throughput Process Development: I—Process Chromatography

      • Anurag S. Rathore, R. Bhambure
      Pages 11-20

    4. High-Throughput Process Development: II—Membrane Chromatography

      • Anurag S. Rathore, S. Muthukumar
      Pages 21-26

    5. Media Selection in Ion Exchange Chromatography in a Single Microplate

      • Charlotte Cabanne, Xavier Santarelli
      Pages 27-33

    6. High-Throughput Screening of Dye-Ligands for Chromatography

      • Sunil Kumar, Narayan S. Punekar
      Pages 35-47

    7. Technical Optimization for the High-Throughput Purification of Antibodies on Automated Liquid Handlers

      • Peter M. Schmidt
      Pages 49-62

  3. Low-Resolution Protein Purification Methods

    1. Front Matter

      Pages 63-63
      Download chapter PDF

    2. Aqueous Two-Phase Systems for Cleanup and Recovery of Enzymes from Plants and Plant-Derived Extracts

      • Oscar Aguilar, Erick Heredia-Olea, Esther Perez-Carrillo, Marco Rito-Palomares
      Pages 65-79

    3. Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins

      • Anurag S. Rathore, R. Bhambure
      Pages 81-91

    4. Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments

      • Ario de Marco
      Pages 93-103

  4. High-Resolution Protein Purification Methods

    1. Front Matter

      Pages 105-105
      Download chapter PDF

    2. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

      • Ana Sofia Pina, Íris L. Batalha, Ana M. G. C. Dias, Ana Cecília A. Roque
      Pages 107-132

    3. Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins

      • Sharon Williams, Phil Morton, Dev Baines
      Pages 133-148

    4. Zbasic: A Purification Tag for Selective Ion-Exchange Recovery

      • My Hedhammar, Johan Nilvebrant, Sophia Hober
      Pages 149-158

    5. An Orthogonal Fusion Tag for Efficient Protein Purification

      • Johan Nilvebrant, Mikael Åstrand, Sophia Hober
      Pages 159-166

    6. Biomimetic Affinity Ligands for Protein Purification

      • Isabel T. Sousa, M. Ângela Taipa
      Pages 167-199

    7. Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification

      • Evangelia G. Chronopoulou, Georgios Premetis, Christina Varotsou, Nikolaos Georgakis, Elisavet Ioannou, Nikolaos E. Labrou
      Pages 201-215

    8. Design of Affinity Chromatography Peptide Ligands Through Combinatorial Peptide Library Screening

      • G. R. Barredo, S. L. Saavedra, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio et al.
      Pages 217-243
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Keywords

About this book

This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry. 


Editors and Affiliations

  • Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

Bibliographic Information

  • Book Title: Protein Downstream Processing

  • Book Subtitle: Design, Development, and Application of High and Low-Resolution Methods

  • Editors: Nikolaos E. Labrou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0775-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0774-9Published: 01 November 2020

  • Softcover ISBN: 978-1-0716-0777-0Published: 01 November 2021

  • eBook ISBN: 978-1-0716-0775-6Published: 31 October 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 511

  • Number of Illustrations: 49 b/w illustrations, 78 illustrations in colour

  • Topics: Protein Science, Chemistry/Food Science, general, Biotechnology, Biomaterials

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