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Methods in Molecular Biology

RNA Tagging

Methods and Protocols

Editors: Heinlein, Manfred (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-0716-0712-1
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About this book

This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization

    Pages 3-21

    Dresselhaus, Thomas (et al.)

  • Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution

    Pages 23-33

    Huang, Kun (et al.)

  • Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells

    Pages 35-49

    Wu, Jingze J. (et al.)

  • Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single-Molecule FISH and Immunofluorescence

    Pages 51-69

    Tutucci, Evelina (et al.)

  • Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More

    Pages 73-102

    Ryckelynck, Michael

Buy this book

eBook 139,99 €
price for Spain (gross)
  • ISBN 978-1-0716-0712-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-0716-0711-4
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
RNA Tagging
Book Subtitle
Methods and Protocols
Editors
  • Manfred Heinlein
Series Title
Methods in Molecular Biology
Series Volume
2166
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0712-1
DOI
10.1007/978-1-0716-0712-1
Hardcover ISBN
978-1-0716-0711-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 490
Number of Illustrations
14 b/w illustrations, 77 illustrations in colour
Topics

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