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Kupffer Cells

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Myriam Aouadi

    1. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Valerio Azzimato

    1. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2164)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. Kupffer Cell and Hepatocyte Isolation from a Single Mouse Liver by Gradient Centrifugation

    • Carolina Oses, Myriam Aouadi
    Pages 1-10

  3. Kupffer Cell Isolation from Human Biopsies

    • Cecilia Morgantini
    Pages 11-13

  4. Liver Macrophage Isolation by Flow Cytometry Sorting

    • André Sulen
    Pages 15-20

  5. Kupffer Cell Protein Isolation and Detection by Western Blot

    • Valerio Azzimato
    Pages 21-26

  6. Kupffer Cell mRNA Sequencing

    • Emelie Barreby, Connie Xu
    Pages 27-44

  7. Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections

    • Adrien Guillot, Chloé Buch, Tony Jourdan
    Pages 45-53

  8. Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages Using 3D Microscopy

    • Otto Strauss, Niklas K. Björkström
    Pages 55-63

  9. Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver

    • Michaela Tencerova
    Pages 65-73

  10. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

    • Qiushi Tang, Gwladys Gernoux, Yangfun Cheng, Terence Flotte, Christian Mueller
    Pages 75-85

  11. Kupffer Cell Characterization by Mass Cytometry

    • Camille Blériot, Shamin Li, Muhammad Faris Bin Mohd Kairi, Evan Newell, Florent Ginhoux
    Pages 87-99

  12. Enzymatic Quantification of Liver Lipids After Folch Extraction

    • Mirko E. Minniti, Osman Ahmed, Matteo Pedrelli
    Pages 101-108

  13. In Vivo Investigation of High-Fat Diet–Induced Hepatic Lipid Dysfunctions

    • Marion Korach-André
    Pages 109-119

  14. An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis

    • Bader Zarrouki, Jeremie Boucher
    Pages 121-127

  15. A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

    • Sijia Yu, Santiago Vernia
    Pages 129-143

  16. Mouse Model of Alcoholic Steatohepatitis

    • Adeline Bertola
    Pages 145-157

  17. Back Matter

    Pages 159-160
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Keywords

About this book

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

  

Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols  aims to ensure successful investigation of this growing field. 

Editors and Affiliations

  • Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Myriam Aouadi, Valerio Azzimato

Bibliographic Information

  • Book Title: Kupffer Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Myriam Aouadi, Valerio Azzimato

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0704-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0703-9Published: 01 July 2020

  • Softcover ISBN: 978-1-0716-0706-0Published: 01 July 2021

  • eBook ISBN: 978-1-0716-0704-6Published: 30 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 160

  • Number of Illustrations: 10 b/w illustrations, 24 illustrations in colour

  • Topics: Cell Biology

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