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Arbuscular Mycorrhizal Fungi

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Nuria Ferrol

    1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, CSIC, Granada, Spain

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  2. Luisa Lanfranco

    1. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2146)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples

    • Dora Trejo-Aguilar, Jacob Banuelos
    Pages 1-18

  3. The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants

    • Ismahen Lalaymia, Stéphane Declerck
    Pages 19-31

  4. A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium

    • Cristiana Sbrana, Alessandra Pepe, Nuria Ferrol, Manuela Giovannetti
    Pages 33-41

  5. Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization

    • Tania Ho-Plágaro, María Isabel Tamayo-Navarrete, José Manuel García-Garrido
    Pages 43-52

  6. Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide

    • Gennaro Carotenuto, Andrea Genre
    Pages 53-59

  7. Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity

    • Pablo Cornejo, Humberto Aponte
    Pages 61-71

  8. Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization

    • Martina Janoušková, Petra Caklová
    Pages 73-91

  9. Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi

    • Dirk Redecker
    Pages 93-97

  10. High-Throughput DNA Sequence-Based Analysis of AMF Communities

    • Íris Marisa Maxaieie Victorino, Andrea Berruti, Alberto Orgiazzi, Samuele Voyron, Valeria Bianciotto, Erica Lumini
    Pages 99-116

  11. Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP)

    • Álvaro López-García
    Pages 117-136

  12. Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi

    • Francesco Venice, Alessandra Salvioli di Fossalunga, Paola Bonfante
    Pages 137-153

  13. Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR

    • Alessandro Silvestri, Jacob Pérez-Tienda, Juan Antonio López-Ráez
    Pages 157-170

  14. Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

    • Raffaella Balestrini, Valentina Fiorilli
    Pages 171-184

  15. Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization

    • Bettina Hause, Natalia Requena
    Pages 185-196

  16. Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast

    • Elisabeth Tamayo, Tamara Gómez-Gallego, Nuria Ferrol
    Pages 197-211

  17. Isotope Labeling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis

    • Carla Cruz-Paredes, Mayra E. Gavito
    Pages 213-222

  18. Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae

    • Ghislaine Recorbet, Pierre-Emmanuel Courty, Daniel Wipf
    Pages 223-238

  19. Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium rhizogenes-Mediated Root Transformation in Medicago truncatula

    • Meike Hartmann, Stefanie Voß, Natalia Requena
    Pages 239-248

  20. Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi

    • Tatsuhiro Ezawa, Hayato Maruyama, Yusuke Kikuchi, Kaede Yokoyama, Chikara Masuta
    Pages 249-254
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Keywords

About this book

This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbiontsin agriculture more effectively.

Editors and Affiliations

  • Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, CSIC, Granada, Spain

    Nuria Ferrol

  • Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy

    Luisa Lanfranco

Bibliographic Information

  • Book Title: Arbuscular Mycorrhizal Fungi

  • Book Subtitle: Methods and Protocols

  • Editors: Nuria Ferrol, Luisa Lanfranco

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0603-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0602-5Published: 16 May 2020

  • Softcover ISBN: 978-1-0716-0605-6Published: 16 May 2021

  • eBook ISBN: 978-1-0716-0603-2Published: 15 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 257

  • Number of Illustrations: 12 b/w illustrations, 36 illustrations in colour

  • Topics: Eukaryotic Microbiology, Microbial Genetics and Genomics

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