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Corneal Regeneration

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Mark Ahearne

    1. Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin, Ireland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2145)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Isolation and Culture of Corneal Stromal Stem Cells

    • Richard M. Nagymihaly, Morten C. Moe, Goran Petrovski
    Pages 1-15

  3. In Vitro Expansion of Corneal Endothelial Cells for Transplantation

    • Kim Santerre, Isabelle Xu, Mathieu Thériault, Stéphanie Proulx
    Pages 17-27

  4. Primary Culture of Cornea-Limbal Epithelial Cells In Vitro

    • Finbarr O’Sullivan
    Pages 29-37

  5. Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant

    • Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.
    Pages 39-49

  6. A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture

    • James W. Foster, Karl J. Wahlin, Shukti Chakravarti
    Pages 51-58

  7. Gene Editing for Corneal Stromal Regeneration

    • Tara Moore, Connie Chao-Shern, Larry DeDionisio, Kathleen A. Christie, M. Andrew Nesbit
    Pages 59-75

  8. Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery

    • Liujiang Song, Jacquelyn J. Bower, Matthew L. Hirsch
    Pages 77-102

  9. The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea

    • Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guérin
    Pages 103-118

  10. Formation of Corneal Stromal-Like Assemblies Using Human Corneal Fibroblasts and Macromolecular Crowding

    • Mehmet Gürdal, Gülinnaz Ercan, Dimitrios I. Zeugolis
    Pages 119-141

  11. Preparation of Dried Amniotic Membrane for Corneal Repair

    • Andrew Hopkinson, Emily R. Britchford, Laura E. Sidney
    Pages 143-157

  12. Fabrication of Corneal Extracellular Matrix-Derived Hydrogels

    • Mark Ahearne, Julia Fernández-Pérez
    Pages 159-168

  13. Synthesis and Application of Collagens for Assembling a Corneal Implant

    • Elle Edin, Fiona Simpson, May Griffith
    Pages 169-183

  14. Development and Validation of a 3D In Vitro Model to Study the Chemotactic Behavior of Corneal Stromal Fibroblasts

    • Evrim Ceren Kabak, Julia Fernández-Pérez, Mark Ahearne
    Pages 185-196

  15. Femtosecond Laser-Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration

    • Neil Lagali, Mehrdad Rafat
    Pages 197-214

  16. The Use of Animal Models to Assess Engineered Corneal Tissue

    • Robert Thomas Brady, Peter W. Madden
    Pages 215-230

  17. X-Ray Diffraction Imaging of Corneal Ultrastructure

    • Keith M. Meek, Andrew J. Quantock, Sally Hayes, James Bell
    Pages 231-247

  18. Correction to: Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant

    • Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.
    Pages C1-C1

  19. Back Matter

    Pages 249-250
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Keywords

About this book

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Editors and Affiliations

  • Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin, Ireland

    Mark Ahearne

Bibliographic Information

  • Book Title: Corneal Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Mark Ahearne

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0599-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0598-1Published: 16 June 2020

  • Softcover ISBN: 978-1-0716-0601-8Published: 16 June 2021

  • eBook ISBN: 978-1-0716-0599-8Published: 15 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 250

  • Number of Illustrations: 23 b/w illustrations, 43 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Ophthalmology, Biomedical Engineering/Biotechnology

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