Methods in Molecular Biology

Corneal Regeneration

Methods and Protocols

Editors: Ahearne, Mark (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0599-8
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Hardcover 176,79 €
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Softcover 124,79 €
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About this book

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Isolation and Culture of Corneal Stromal Stem Cells

    Pages 1-15

    Nagymihaly, Richard M. (et al.)

  • In Vitro Expansion of Corneal Endothelial Cells for Transplantation

    Pages 17-27

    Santerre, Kim (et al.)

  • Primary Culture of Cornea-Limbal Epithelial Cells In Vitro

    Pages 29-37

    O’Sullivan, Finbarr

  • Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant

    Pages 39-49

    López-Paniagua, Marina (et al.)

  • A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture

    Pages 51-58

    Foster, James W. (et al.)

Buy this book

eBook 99,99 €
price for Spain (gross)
  • ISBN 978-1-0716-0599-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-0716-0598-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 124,79 €
price for Spain (gross)
  • ISBN 978-1-0716-0601-8
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Corneal Regeneration
Book Subtitle
Methods and Protocols
Editors
  • Mark Ahearne
Series Title
Methods in Molecular Biology
Series Volume
2145
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0599-8
DOI
10.1007/978-1-0716-0599-8
Hardcover ISBN
978-1-0716-0598-1
Softcover ISBN
978-1-0716-0601-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 250
Number of Illustrations
23 b/w illustrations, 43 illustrations in colour
Topics