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Fasciola hepatica

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Martin Cancela

    1. Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

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  2. Gabriela Maggioli

    1. Unidad de Biología Parasitaria, Instituto de Higiene Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2137)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Maintenance of Life Cycle Stages of Fasciola hepatica in the Laboratory

    • Valeria Gayo, Martin Cancela, Daniel Acosta
    Pages 1-14

  3. Microscopical Techniques to Analyze the Hepatic and Peritoneal Changes Caused by Fasciola hepatica Infection

    • M. T. Ruiz-Campillo, V. Molina-Hernández, J. Pérez, I. L. Pacheco, R. Pérez, A. Escamilla et al.
    Pages 15-25

  4. Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

    • Eduardo de la Torre-Escudero, Mark W. Robinson
    Pages 27-36

  5. Isolation and Analysis of Fasciola hepatica Extracellular Vesicles

    • Alicia Galiano, Maria Teresa Minguez, Christian M. Sánchez, Antonio Marcilla
    Pages 37-50

  6. Cloning and Heterologous Expression of Protein-Coding Sequences in Escherichia coli

    • Martin Cancela, Gabriela Maggioli
    Pages 51-66

  7. Gene Silencing in the Liver Fluke Fasciola hepatica: RNA Interference

    • Gabriel Rinaldi, Nicolás Dell’Oca, Estela Castillo, José F. Tort
    Pages 67-92

  8. Analysis of Gene Expression in Fasciola hepatica Juveniles and Adults by In Situ Hybridization

    • Estela Castillo, Uriel Koziol
    Pages 93-105

  9. Evasion of Host Immunity During Fasciola hepatica Infection

    • Robin J. Flynn, Mayowa Musah-Eroje
    Pages 107-115

  10. Immunomodulatory Effect of Fasciola hepatica Excretory–Secretory Products on Macrophages

    • Lorena Guasconi, Marianela C. Serradell, Diana T. Masih, Laura S. Chiapello
    Pages 117-131

  11. Study of Eosinophil Apoptosis Induced by Fasciola hepatica Excretory–Secretory Products

    • Marianela C. Serradell, Lorena Guasconi, Laura Cervi, Laura S. Chiapello, Diana T. Masih
    Pages 133-148

  12. Purification of Native Fasciola hepatica Fatty Acid-Binding Protein and Induction of Alternative Activation of Human Macrophages

    • Olgary Figueroa-Santiago, Ana Espino
    Pages 149-169

  13. Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory–Secretory Products with Human Monocyte Cell Line

    • Olgary Figueroa-Santiago, Ana Espino
    Pages 171-180

  14. Evaluation of the Immune Regulatory Properties of Dendritic Cells During Fasciola hepatica Infection

    • Teresa Freire
    Pages 181-190

  15. Design of a Peptide-Carrier Vaccine Based on the Highly Immunogenic Fasciola hepatica Leucine Aminopeptidase

    • Cecilia Salazar, José F. Tort, Carlos Carmona
    Pages 191-204

  16. Liver Fluke Vaccine Assessment in Cattle

    • Gabriela Maggioli, Cecilia Salazar, Federico Fossa, Carlos Carmona
    Pages 205-212

  17. Testing Albendazole Resistance in Fasciola hepatica

    • Luis I. Alvarez, María Martinez Valladares, Candela Canton, Carlos E. Lanusse, Laura Ceballos
    Pages 213-220

  18. Drug Targets: Screening for Small Molecules that Inhibit Fasciola hepatica Enzymes

    • Florencia Ferraro, Mauricio A. Cabrera, Guzmán I. Álvarez, Ileana Corvo
    Pages 221-231

  19. Correction to: Fasciola hepatica: Methods and Protocols

    • Martin Cancela, Gabriela Maggioli
    Pages C1-C1

  20. Back Matter

    Pages 233-235
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Keywords

About this book

This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry,immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.

Editors and Affiliations

  • Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Martin Cancela

  • Unidad de Biología Parasitaria, Instituto de Higiene Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay

    Gabriela Maggioli

Bibliographic Information

  • Book Title: Fasciola hepatica

  • Book Subtitle: Methods and Protocols

  • Editors: Martin Cancela, Gabriela Maggioli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0475-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0474-8Published: 14 May 2020

  • Softcover ISBN: 978-1-0716-0477-9Published: 14 May 2021

  • eBook ISBN: 978-1-0716-0475-5Published: 13 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 235

  • Number of Illustrations: 13 b/w illustrations, 22 illustrations in colour

  • Topics: Immunology, Infectious Diseases, Parasitology

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