Skip to main content
SpringerLink
Log in

Leptospira spp.

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Nobuo Koizumi

    1. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Mathieu Picardeau

    1. Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2134)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (25 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Isolation and Culture of Leptospira from Clinical and Environmental Samples

    • Cyrille Goarant, Dominique Girault, Roman Thibeaux, Marie-Estelle Soupé-Gilbert
    Pages 1-9

  3. Core Genome Multi-locus Sequence Typing Analyses of Leptospira spp. Using the Bacterial Isolate Genome Sequence Database

    • Linda Grillová, Mathieu Picardeau
    Pages 11-21

  4. Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira

    • Dominique Girault, Malia Kainiu, Emilie Barsac, Roman Thibeaux, Cyrille Goarant
    Pages 23-29

  5. Cell Enumeration of Leptospira by Flow Cytometry

    • Célia Fontana, Jean Reyes
    Pages 31-40

  6. The Single-Step Method of RNA Purification Applied to Leptospira

    • Crispin Zavala-Alvarado, Nadia Benaroudj
    Pages 41-51

  7. Purification of LPS from Leptospira

    • Delphine Bonhomme, Catherine Werts
    Pages 53-65

  8. Isolation, Purification, and Characterization of Leptophages

    • Olivier Schiettekatte, Pascale Bourhy
    Pages 67-75

  9. Creating a Library of Random Transposon Mutants in Leptospira

    • Christopher J. Pappas, Hui Xu, Md A. Motaleb
    Pages 77-96

  10. Transposon Sequencing in Leptospira interrogans

    • Kristel Lourdault, James Matsunaga
    Pages 97-107

  11. Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically Inactive Cas9 (dCas9)

    • Luis Guilherme Virgílio Fernandes, Ana Lucia Tabet Oller Nascimento
    Pages 109-122

  12. Leptospira spp. Toolbox for Chemotaxis Assay

    • Ambroise Lambert
    Pages 123-130

  13. In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography

    • Akihiro Kawamoto
    Pages 131-138

  14. Measurement of the Cell-Body Rotation of Leptospira

    • Shuichi Nakamura
    Pages 139-148

  15. In Vivo Imaging of Bioluminescent Leptospires

    • Frédérique Vernel-Pauillac, Catherine Werts
    Pages 149-160

  16. Cell Monolayer Translocation Assay

    • Elsio A. Wunder Jr.
    Pages 161-170

  17. Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction

    • Aline F. Teixeira, Ana L. T. O. Nascimento
    Pages 171-185

  18. Complement Resistance Assays

    • Lourdes Isaac, Angela Silva Barbosa
    Pages 187-198

  19. Evaluation of Intracellular Trafficking in Macrophages

    • Claudia Toma, Toshihiko Suzuki
    Pages 199-206

  20. Biofilm Formation and Quantification Using the 96-Microtiter Plate

    • Roman Thibeaux, Malia Kainiu, Cyrille Goarant
    Pages 207-214
Back to top

Keywords

About this book

This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.  


Reviews


Editors and Affiliations

  • Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan

    Nobuo Koizumi

  • Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France

    Mathieu Picardeau

Bibliographic Information

  • Book Title: Leptospira spp.

  • Book Subtitle: Methods and Protocols

  • Editors: Nobuo Koizumi, Mathieu Picardeau

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0459-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0458-8Published: 07 July 2020

  • Softcover ISBN: 978-1-0716-0461-8Published: 07 July 2021

  • eBook ISBN: 978-1-0716-0459-5Published: 07 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 291

  • Number of Illustrations: 10 b/w illustrations, 56 illustrations in colour

  • Topics: Microbiology, Infectious Diseases

Publish with us

Policies and ethics

Back to top

Search

Navigation