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Methods in Molecular Biology
cover

Expressed Protein Ligation

Methods and Protocols

Editors: Vila-Perelló, Miquel (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 139,99 €
price for Spain (gross)
  • ISBN 978-1-0716-0434-2
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Hardcover 176,79 €
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About this book

This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Methods and Applications of Expressed Protein Ligation

    Pages 1-13

    Wang, Zhipeng A. (et al.)

  • Protein Splicing: From the Foundations to the Development of Biotechnological Applications

    Pages 15-29

    Romero-Casañas, Alejandro (et al.)

  • Identification, Characterization, and Optimization of Split Inteins

    Pages 31-54

    Shah, Neel H. (et al.)

  • Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins

    Pages 55-73

    Zhao, Jing (et al.)

  • Expressed Protein Ligation: General Experimental Protocols

    Pages 75-117

    Diaz, Joshua (et al.)

Buy this book

eBook 139,99 €
price for Spain (gross)
  • ISBN 978-1-0716-0434-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Expressed Protein Ligation
Book Subtitle
Methods and Protocols
Editors
  • Miquel Vila-Perelló
Series Title
Methods in Molecular Biology
Series Volume
2133
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0434-2
DOI
10.1007/978-1-0716-0434-2
Hardcover ISBN
978-1-0716-0433-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 362
Number of Illustrations
28 b/w illustrations, 67 illustrations in colour
Topics

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