Stem Cell Nanotechnology

1. Front Matter

   Pages i-xi

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2. Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films Under Flow Conditions

   • Anne-Sophie Mertgen, Markus Rottmar, Lukas Weidenbacher, Anne Géraldine Guex

   Pages 1-13

3. Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation

   • Muge Anil-Inevi, Esra Yilmaz, Oyku Sarigil, H. Cumhur Tekin, Engin Ozcivici

   Pages 15-25

4. Fluorescent Nanoclusters for Imaging of Cells/Stem Cells

   • Fatemeh Molaabasi, Behnam Hajipour-Verdom, Mozhgan Alipour, Seyed Morteza Naghib

   Pages 27-37

5. Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Electrospun Nanofibers

   • Hasham S. Sofi, Nisar Ahmad Khan, Faheem A. Sheikh

   Pages 39-46

6. Preparation and Characterization of Simvastatin Nanocapsules: Encapsulation of Hydrophobic Drugs in Calcium Alginate

   • Mazaher Ahmadi, Tayyebeh Madrakian, Saeid Ghavami

   Pages 47-56

7. Multifunctional Magnetic Nanoparticles-Labeled Mesenchymal Stem Cells for Hyperthermia and Bioimaging Applications

   • Sadegh Ghorbanzade, Seyed Morteza Naghib, Ali Sadr, Fatemeh Sadat Fateminia, Ali Ghaffarinejad, Keivan Majidzadeh-A et al.

   Pages 57-72

8. Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

   • Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Sivakumar Arumugam, Selvamani Palaniswamy

   Pages 73-75

9. Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles

   • Taha Umair Wani, Hasham S. Sofi, Nisar Ahmad Khan, Faheem A. Sheikh

   Pages 77-84

10. Methodology Involved in the Osteogenic Differentiation of Mesenchymal Stem Cells on Chitosan–Collagen Nanofibers Incorporated with Titanium Dioxide Nanoparticles

    • Roqia Ashraf, Hasham S. Sofi, Faheem A. Sheikh

    Pages 85-94

11. Experimental Protocol for Culture and Differentiation of Osteoblasts on 3D Abode Using Nanofiber Scaffolds

    • Hasham S. Sofi, Roqia Ashraf, Faheem A. Sheikh

    Pages 95-108

12. Experimental Protocol of MSC Differentiation into Neural Lineage for Nerve Tissue Regeneration Using Polymeric Scaffolds

    • Roqia Ashraf, Hasham S. Sofi, Faheem A. Sheikh

    Pages 109-117

13. Fabrication and Electrospinning of 3D Biodegradable Poly-l-Lactic Acid (PLLA) Nanofibers for Clinical Application

    • Balasundari Ramesh, Kotturathu Mammen Cherian, Adegbenro Omotuyi John Fakoya

    Pages 119-128

14. Differentiation of Menstrual Blood Stem Cells into Keratinocyte-Like Cells on Bilayer Nanofibrous Scaffold

    • Shaghayegh Arasteh, Sara Katebifar, Reza Shirazi, Somaieh Kazemnejad

    Pages 129-156

15. Dental Pulp Stem Cells in Customized 3D Nanofibrous Scaffolds for Regeneration of Peripheral Nervous System

    • Siddhartha Das, Jayesh R. Bellare

    Pages 157-166

16. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell

    • Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Dinh Toi Chu, Sivakumar Arumugam

    Pages 167-172

17. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell

    • Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Dinh Toi Chu

    Pages 173-179

18. Nanoscaled Materials for Drug Delivery into Cells/Stem Cells

    • Sadegh Ghorbanzade, Seyed Morteza Naghib

    Pages 181-192

19. Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease

    • Nareshwaran Gnanasegaran, Deepak B. Thimiri Govinda Raj, Sivakumar Arumugam

    Pages 193-196

20. Detection and Monitoring of Stem Cell Differentiation Using Nanotechnology

    • Esfandyar Askari, Seyed Morteza Naghib

    Pages 197-204

This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols, Second Edition serves a valuable tool for experts and novices alike in the vital field of stem cell research.

• Regulatory mechanisms
• Nanofiber scaffolds
• Stem cell biology
• Nanoparticles
• Nanoscaled materials

• Ottawa, Canada

  Kursad Turksen

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