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Springer Protocols Handbooks
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Characterization of Plant Viruses

Methods and Protocols

Authors: Bhat, Alangar Ishwara, Rao, Govind Pratap

  • Includes comprehensive and  complete practical manual containing protocols for identification, characterisation and detection of viruses infecting plants based on biological, physico-chemical, serological and nucleic acid properties of the virus 
  • Provides all recent methods in virus identification including lateral flow device, loop-mediated isothermal amplification, rolling circle amplification, next generation sequencing approaches and microarray 
  • Serves as a single source of information and methods of preparation of all reagents required for Plant Virology works
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eBook 149,79 €
price for Spain (gross)
  • ISBN 978-1-0716-0334-5
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Hardcover 187,19 €
price for Spain (gross)
  • ISBN 978-1-0716-0333-8
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  • Immediate ebook access, if available*, with your print order
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About this book

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.


About the authors

Dr Alangar Ishwara Bhat

Alangar Ishwara Bhat is currently working as a Principal Scientist at the ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India. Having obtained his PhD from the ICAR-Indian Agricultural Research Institute, New Delhi, he has 28 years of research experience in Plant Virology and has contributed significantly to the identification, characterization, diagnostics and management of viruses. Dr Bhat has been a visiting scientist at the University of Georgia, USA and at the Food and Environment Research Agency, UK. He has handled ten externally funded projects from the Government of India, authored 89 publications in reputed refereed journals, edited two books, and contributed 28 book chapters and three technical bulletins. He is the chief editor of the Journal of Spices and Aromatic Crops and a section editor of VirusDisease. His work has been recognized with several national awards.

Dr Govind Pratap Rao

Govind Pratap Rao is a Principal Scientist (Plant Pathology) at the Indian Agricultural Research Institute, New Delhi. He received his PhD in Plant Virology from Gorakhpur University in 1986 and pursued his postdoctoral research at the University of Urbana-Champaign, Illinois, USA (1994) and at Cedex, Montpellier, France (1998).  Dr Rao has 32 years of research experience, and has made significant contributions to the characterization of plant viruses and phytoplasmas. He has over 150 research articles, and 25 and authored and edited books to his credit.  His work has been recognized with several prestigious national and international awards. Dr Rao is also Secretary General of the Indian Virological Society, New Delhi and a member of several prestigious international scientific societies. In addition, he has travelled to more than 30 countries as a visiting scientist, expert, for invited talks, research training, panel discussions and conferences. 

Table of contents (50 chapters)

Table of contents (50 chapters)
  • Glasshouse for Maintenance of Virus and Insect Culture

    Pages 1-5

    Bhat, Alangar Ishwara (et al.)

  • Symptoms of Virus-Infected Plants

    Pages 7-21

    Bhat, Alangar Ishwara (et al.)

  • Isolation and Diagnosis of Virus Through Indicator Hosts

    Pages 23-27

    Bhat, Alangar Ishwara (et al.)

  • Host Range of Viruses

    Pages 29-31

    Bhat, Alangar Ishwara (et al.)

  • Physico-chemical Properties of Virus in Crude Sap

    Pages 33-38

    Bhat, Alangar Ishwara (et al.)

Buy this book

eBook 149,79 €
price for Spain (gross)
  • ISBN 978-1-0716-0334-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 187,19 €
price for Spain (gross)
  • ISBN 978-1-0716-0333-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Characterization of Plant Viruses
Book Subtitle
Methods and Protocols
Authors
Series Title
Springer Protocols Handbooks
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0334-5
DOI
10.1007/978-1-0716-0334-5
Hardcover ISBN
978-1-0716-0333-8
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XXI, 552
Number of Illustrations
62 b/w illustrations, 99 illustrations in colour
Topics

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