Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2116)
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Table of contents (48 protocols)
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Front Matter
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Isolation, Culturing, and Genetic Manipulation of Trypanosomatids
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Front Matter
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Trypanosomatid Genome-Wide Analyses
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Front Matter
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Molecular Biology and Genetics
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Front Matter
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About this book
This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases.
Chapters 14, 15, 16, 23, 24, 30, and 48 are open access under a CC BY 4.0 license.
Editors and Affiliations
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School of Biological Sciences, University of Edinburgh, Edinburgh, UK
Paul A. M. Michels
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School of Applied Sciences, University of Huddersfield, Huddersfield, UK
Michael L. Ginger
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Faculty of Biology, Technion–Israel Institute of Technology, Haifa, Israel
Dan Zilberstein
Bibliographic Information
Book Title: Trypanosomatids
Book Subtitle: Methods and Protocols
Editors: Paul A. M. Michels, Michael L. Ginger, Dan Zilberstein
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0294-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0293-5Published: 28 March 2020
Softcover ISBN: 978-1-0716-0296-6Published: 28 March 2021
eBook ISBN: 978-1-0716-0294-2Published: 27 March 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXI, 823
Number of Illustrations: 50 b/w illustrations, 101 illustrations in colour
Topics: Infectious Diseases, Human Genetics