Methods in Molecular Biology

RNA Interference and CRISPR Technologies

Technical Advances and New Therapeutic Opportunities

Editors: Sioud, Mouldy (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key note and implementation advice from the experts
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eBook 179,99 €
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  • ISBN 978-1-07-160290-4
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Hardcover 228,79 €
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About this book

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. 

Table of contents (27 chapters)

Table of contents (27 chapters)
  • RNA and CRISPR Interferences: Past, Present, and Future Perspectives

    Pages 1-22

    Sioud, Mouldy

  • Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents

    Pages 23-55

    Lennox, Kim A. (et al.)

  • Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo

    Pages 57-77

    Chernikov, Ivan V. (et al.)

  • Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy

    Pages 79-90

    Lee, Seung Koo (et al.)

  • PAMAM Dendrimers as a Delivery System for Small Interfering RNA

    Pages 91-106

    Kheiriabad, Shiva (et al.)

Buy this book

eBook 179,99 €
price for Spain (gross)
  • ISBN 978-1-07-160290-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 228,79 €
price for Spain (gross)
  • ISBN 978-1-07-160289-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
RNA Interference and CRISPR Technologies
Book Subtitle
Technical Advances and New Therapeutic Opportunities
Editors
  • Mouldy Sioud
Series Title
Methods in Molecular Biology
Series Volume
2115
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160290-4
DOI
10.1007/978-1-0716-0290-4
Hardcover ISBN
978-1-07-160289-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 486
Number of Illustrations
22 b/w illustrations, 48 illustrations in colour
Topics