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Methods in Molecular Biology

Cytoskeleton Dynamics

Methods and Protocols

Editors: Maiato, Helder (Ed.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook 139,99 €
price for Spain (gross)
  • ISBN 978-1-07-160219-5
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-07-160218-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers.


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Purification of Mammalian Tubulins and Tubulin-Associated Proteins Using a P2A-Based Expression System

    Pages 1-17

    Yu, Nuo (et al.)

  • Expression and Purification of Microtubule-Associated Proteins from HEK293T Cells for In Vitro Reconstitution

    Pages 19-26

    Hua, Shasha (et al.)

  • In Vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule-Severing Enzymes Katanin and Spastin

    Pages 27-38

    Vemu, Annapurna (et al.)

  • In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy

    Pages 39-51

    Spector, Jeffrey O. (et al.)

  • In Vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Filaments in Emulsion Droplets

    Pages 53-75

    Vendel, Kim J. A. (et al.)

Buy this book

eBook 139,99 €
price for Spain (gross)
  • ISBN 978-1-07-160219-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-07-160218-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Cytoskeleton Dynamics
Book Subtitle
Methods and Protocols
Editors
  • Helder Maiato
Series Title
Methods in Molecular Biology
Series Volume
2101
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160219-5
DOI
10.1007/978-1-0716-0219-5
Hardcover ISBN
978-1-07-160218-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 390
Number of Illustrations
12 b/w illustrations, 72 illustrations in colour
Topics