Plant Epigenetics and Epigenomics
Methods and Protocols
Editors: Spillane, Charles, McKeown, Peter (Eds.)
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementations advice from the experts
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- About this book
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This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
- Table of contents (18 chapters)
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An Overview of Current Research in Plant Epigenetic and Epigenomic Phenomena
Pages 3-13
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Approaches to Whole-Genome Methylome Analysis in Plants
Pages 15-31
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Understanding DNA Methylation Patterns in Wheat
Pages 33-46
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MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation
Pages 47-64
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Plant-RRBS: DNA Methylome Profiling Adjusted to Plant Genomes, Utilizing Efficient Endonuclease Combinations, for Multi-Sample Studies
Pages 65-80
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Table of contents (18 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Epigenetics and Epigenomics
- Book Subtitle
- Methods and Protocols
- Editors
-
- Charles Spillane
- Peter McKeown
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2093
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0179-2
- DOI
- 10.1007/978-1-0716-0179-2
- Hardcover ISBN
- 978-1-0716-0178-5
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XI, 272
- Number of Illustrations
- 12 b/w illustrations, 39 illustrations in colour
- Topics
