Skip to main content
SpringerLink
Log in
Book cover

Malaria Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Denise L. Doolan

    1. Malaria Program, Naval Medical Research Center, Silver Spring, USA
      Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 72)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (57 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Assessing Risk of Infection and Severity of Disease

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Vector Incrimination and Entomological Inoculation Rates

      • John C. Beier
      Pages 3-11

    3. Epidemiological Measures of Risk of Malaria

      • J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, Mazie J. Barcus
      Pages 13-22

  3. Laboratory Models

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Maintenance of the Plasmodium berghei Life Cycle

      • Robert E. Sinden, Geoff A. Butcher, A. L. Beetsma
      Pages 25-40

    3. Mouse Models for Pre-Erythrocytic-Stage Malaria

      • Laurent Rénia, Elodie Belnoue, Irène Landau
      Pages 41-55

    4. Mouse Models for Erythrocytic-Stage Malaria

      • Latifu A. Sanni, Luis F. Fonseca, Jean Langhorne
      Pages 57-76

    5. Nonhuman Primate Models

      • William E. Collins
      Pages 77-84

    6. Nonhuman Primate Models

      • William E. Collins
      Pages 85-92

  4. Diagnosis and Typing

    1. Front Matter

      Pages 93-93
      Download chapter PDF

    2. Vector Analysis

      • John C. Beier
      Pages 95-101

    3. Genotyping of Plasmodium spp.

      • Georges Snounou
      Pages 103-116

    4. Genotyping of Plasmodium falciparum

      • Ingrid Felger, Hans-Peter Beck
      Pages 117-129

    5. Microsatellite Analysis in Plasmodium falciparum

      • Xin-zhuan Su, Michael T. Ferdig
      Pages 131-136

    6. Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR

      • Adam A. Witney, Robert M. Anthony, Daniel J. Carucci
      Pages 137-139

    7. Quantitation of Liver-Stage Parasites by Competitive RT-PCR

      • Kyle C. McKenna, Marcelo R. S. Briones
      Pages 141-148

  5. Molecular Biology Techniques

    1. Front Matter

      Pages 149-149
      Download chapter PDF

    2. Extraction and Purification of Plasmodium Total RNA

      • Till Voss
      Pages 151-157

    3. Extraction and Purification of Plasmodium Parasite DNA

      • Hans-Peter Beck
      Pages 159-163
Back to top

Keywords

About this book

The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.

Reviews

"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International

"This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology

Editors and Affiliations

  • Malaria Program, Naval Medical Research Center, Silver Spring, USA

    Denise L. Doolan

  • Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, USA

    Denise L. Doolan

Bibliographic Information

  • Book Title: Malaria Methods and Protocols

  • Editors: Denise L. Doolan

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592592716

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-823-3Published: 28 June 2002

  • Softcover ISBN: 978-1-61737-205-6Published: 09 November 2010

  • eBook ISBN: 978-1-59259-271-5Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 631

  • Topics: Infectious Diseases

Publish with us

Policies and ethics

Back to top

Search

Navigation