Plant Membrane Proteomics

1. Front Matter

   Pages i-xii

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2. Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis

   • Bronwyn J. Barkla

   Pages 1-12

3. Isolation and Purity Assessment of Membranes from Norway Spruce

   • Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt et al.

   Pages 13-39

4. Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis

   • Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty

   Pages 41-55

5. Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis

   • Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána et al.

   Pages 57-79

6. Isolation, Purity Assessment, and Proteomic Analysis of Nuclei

   • Setsuko Komatsu

   Pages 81-90

7. Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient

   • Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko et al.

   Pages 91-105

8. Analyzing the Vacuolar Membrane (Tonoplast) Proteome

   • Miwa Ohnishi, Katsuhisa Yoshida, Tetsuro Mimura

   Pages 107-116

9. Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies

   • Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphné Seigneurin-Berny, Norbert Rolland

   Pages 117-136

10. Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria

    • Ann Magnuson, Tanai Cardona

    Pages 137-145

11. Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development

    • Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas et al.

    Pages 147-162

12. Sample Preparation for Analysis of the Plant Mitochondrial Membrane Proteome

    • Christine Schikowsky, Beate Thal, Hans-Peter Braun, Holger Eubel

    Pages 163-183

13. Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS

    • Bin Li, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura

    Pages 185-194

14. Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis

    • Anna M. Jozefowicz, Andrea Matros, Katja Witzel, Hans-Peter Mock

    Pages 195-204

15. Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses

    • Małgorzata Janicka, Anna Wdowikowska, Grażyna Kłobus

    Pages 205-215

16. Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters

    • Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood

    Pages 217-234

17. MSE for Label-Free Absolute Protein Quantification in Complex Proteomes

    • Stefan Helm, Sacha Baginsky

    Pages 235-247

18. Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON

    • Rainer Schwacke, Ulf-Ingo Flügge

    Pages 249-259

19. VANTED: A Tool for Integrative Visualization and Analysis of -Omics Data

    • Anja Hartmann, Anna Maria Jozefowicz

    Pages 261-278

20. Back Matter

    Pages 279-280

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This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Practical and thorough, Plant Membrane Proteomics: Methodsand Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.

• 2-D gel electrophoresis
• liquid chromatography (LC)-based peptide separation
• mass spectrometry (MS)
• plasma membranes
• plant cellular networks
• Arabidopsis
• subcellular fractionation

• Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

  Hans-Peter Mock, Andrea Matros

• Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany

  Katja Witzel

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