Diabetic Nephropathy

1. Front Matter

   Pages i-xiii

   PDF

2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. Diabetic Nephropathy: An Overview

      • Manpreet K. Sagoo, Luigi Gnudi

      Pages 3-7

3. Experimental Models to Study Diabetic Nephropathy

   1. Front Matter

      Pages 9-9

      PDF

   2. Modeling Podocyte Biology Using Drosophila Nephrocytes

      • Paul S. Hartley, Richard J. Coward

      Pages 11-24

   3. A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros

      • Maria Kolatsi-Joannou, Daniel Osborn

      Pages 25-39

   4. Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model

      • Carolynn Cairns, Bryan Conway

      Pages 41-52

   5. Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

      • Elisavet Vasilopoulou

      Pages 53-59

4. Techniques to Image the Diabetic Kidney

   1. Front Matter

      Pages 61-61

      PDF

   2. Histological Examination of the Diabetic Kidney

      • Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti

      Pages 63-87

   3. Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy

      • Kathryn E. White

      Pages 89-102

   4. Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy

      • Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding

      Pages 103-126

5. Assessing the Function and Pathophysiological Properties of the Diabetic Kidney

   1. Front Matter

      Pages 127-127

      PDF

   2. Transcutaneous Measurement of Glomerular Filtration Rate in Rodents

      • Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz

      Pages 129-137

   3. An In Vitro Method to Analyze Glucose Uptake in Podocytes

      • Abigail C. Lay, Richard J. Coward

      Pages 139-143

   4. Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro

      • Raina D. Ramnath, Simon C. Satchell

      Pages 145-151

   5. Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy

      • Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann

      Pages 153-173

   6. Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease

      • Karina Barreiro, Tobias B. Huber, Harry Holthofer

      Pages 175-188

   7. Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy

      • Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires

      Pages 189-201

6. Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney

   1. Front Matter

      Pages 203-203

      PDF

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and todevelop treatments for this condition.

• Diabetes mellitus
• Renal failure
• Kidney imaging
• Pathophysiology
• Regenerative medicine
• Diabetic kidney disease
• Experimental models.
• diabetes

• Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research Excellence, King’s College London, London, UK

  Luigi Gnudi

• Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child Health, University College London, London, UK

  David A. Long

Policies and ethics