Methods in Molecular Biology

Cryptosporidium

Methods and Protocols

Editors: Mead, Jan R., Arrowood, Michael J. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-9748-0
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About this book

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Cryptosporidium Diagnostic Assays: Microscopy

    Pages 1-10

    Robinson, Guy (et al.)

  • Cryptosporidium Diagnostic Assays: Molecular Detection

    Pages 11-22

    Robinson, Guy (et al.)

  • Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

    Pages 23-41

    Kahler, Amy M. (et al.)

  • Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation

    Pages 43-59

    Arrowood, Michael J.

  • Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

    Pages 61-85

    Priest, Jeffrey W. (et al.)

Buy this book

eBook £87.50
price for United Kingdom (gross)
  • ISBN 978-1-4939-9748-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover £149.99
price for United Kingdom (gross)
Softcover £109.99
price for United Kingdom (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Cryptosporidium
Book Subtitle
Methods and Protocols
Editors
  • Jan R. Mead
  • Michael J. Arrowood
Series Title
Methods in Molecular Biology
Series Volume
2052
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9748-0
DOI
10.1007/978-1-4939-9748-0
Hardcover ISBN
978-1-4939-9747-3
Softcover ISBN
978-1-4939-9750-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 407
Number of Illustrations
22 b/w illustrations, 71 illustrations in colour
Topics