Get 40% off of select print and eBooks in Engineering & Materials Science!

Methods in Molecular Biology

Lipid-Activated Nuclear Receptors

Methods and Protocols

Editors: Gage, Matthew C., Pineda-Torra, Inés (Eds.)

Free Preview
  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
see more benefits

Buy this book

eBook £103.50
price for United Kingdom (gross)
  • ISBN 978-1-4939-9130-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover £129.99
price for United Kingdom (gross)
  • ISBN 978-1-4939-9129-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS

    Pages 1-14

    Magomedova, Lilia (et al.)

  • A Stable Luciferase Reporter System to Characterize LXR Regulation by Oxysterols and Novel Ligands

    Pages 15-32

    Hutchinson, Samantha A. (et al.)

  • Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages

    Pages 33-48

    Julla, Jean-Baptiste (et al.)

  • Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages

    Pages 49-57

    Barreby, Emelie (et al.)

  • Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity

    Pages 59-73

    Alonso-Herranz, Laura (et al.)

Buy this book

eBook £103.50
price for United Kingdom (gross)
  • ISBN 978-1-4939-9130-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover £129.99
price for United Kingdom (gross)
  • ISBN 978-1-4939-9129-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Lipid-Activated Nuclear Receptors
Book Subtitle
Methods and Protocols
Editors
  • Matthew C. Gage
  • Inés Pineda-Torra
Series Title
Methods in Molecular Biology
Series Volume
1951
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9130-3
DOI
10.1007/978-1-4939-9130-3
Hardcover ISBN
978-1-4939-9129-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 220
Number of Illustrations
8 b/w illustrations, 27 illustrations in colour
Topics