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Lipid-Activated Nuclear Receptors

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Matthew C. Gage

    1. Division of Medicine, Centre for Cardiometabolic Medicine, University College London, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Inés Pineda-Torra

    1. Division of Medicine, Centre for Cardiometabolic Medicine, University College London, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1951)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS

    • Lilia Magomedova, Carolyn L. Cummins
    Pages 1-14

  3. A Stable Luciferase Reporter System to Characterize LXR Regulation by Oxysterols and Novel Ligands

    • Samantha A. Hutchinson, James L. Thorne
    Pages 15-32

  4. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages

    • Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, Fawaz Alzaid
    Pages 33-48

  5. Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages

    • Emelie Barreby, André Sulen, Myriam Aouadi
    Pages 49-57

  6. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity

    • Laura Alonso-Herranz, Jesús Porcuna, Mercedes Ricote
    Pages 59-73

  7. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells

    • Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, Antonio Castrillo
    Pages 75-85

  8. Dual Cross-Linking Chromatin Immunoprecipitation Protocol for Next-Generation Sequencing (ChIPseq) in Macrophages

    • David A. Rollins, Inez Rogatsky
    Pages 87-98

  9. Analysis of LXR Nuclear Receptor Cistrome Through ChIP-Seq Data Bioinformatics

    • Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, Antonio Castrillo
    Pages 99-109

  10. Quantifying Cellular Cholesterol Efflux

    • Sabrina Robichaud, Mireille Ouimet
    Pages 111-133

  11. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection

    • Estibaliz Glaría, Jonathan Matalonga, Annabel F. Valledor
    Pages 135-141

  12. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency

    • Matthew C. Gage
    Pages 143-152

  13. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution

    • Ada Weinstock, Edward A. Fisher
    Pages 153-165

  14. Preparation of Frozen Liver Tissues for Integrated Omics Analysis

    • Ning Liang, Rongrong Fan, Saioa Goñi, Eckardt Treuter
    Pages 167-178

  15. Identification of Nuclear Receptor Targets by Chromatin Immunoprecipitation in Fatty Liver

    • Natalia Becares, Inés Pineda-Torra
    Pages 179-188

  16. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo

    • Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels et al.
    Pages 189-207

  17. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry

    • Kirsty E. Waddington, Inés Pineda-Torra, Elizabeth C. Jury
    Pages 209-216

  18. Back Matter

    Pages 217-220
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Keywords

About this book

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.

Editors and Affiliations

  • Division of Medicine, Centre for Cardiometabolic Medicine, University College London, London, UK

    Matthew C. Gage, Inés Pineda-Torra

Bibliographic Information

  • Book Title: Lipid-Activated Nuclear Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew C. Gage, Inés Pineda-Torra

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9130-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9129-7Published: 04 March 2019

  • eBook ISBN: 978-1-4939-9130-3Published: 01 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 220

  • Number of Illustrations: 8 b/w illustrations, 27 illustrations in colour

  • Topics: Receptors, Lipidology

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