Overview
- Includes cutting-edge techniques for the study of insect genomics
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1858)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
Keywords
About this book
This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.
Editors and Affiliations
Bibliographic Information
Book Title: Insect Genomics
Book Subtitle: Methods and Protocols
Editors: Susan J. Brown, Michael E. Pfrender
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8775-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8774-0Published: 10 November 2018
Softcover ISBN: 978-1-4939-9389-5Published: 10 December 2019
eBook ISBN: 978-1-4939-8775-7Published: 09 November 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 237
Number of Illustrations: 11 b/w illustrations, 23 illustrations in colour
Topics: Human Genetics