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Metabolic Signaling

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sarah-Maria Fendt

    1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Sophia Y. Lunt

    1. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1862)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. A Protocol to Compare Methods for Untargeted Metabolomics

    • Lingjue Wang, Fuad J. Naser, Jonathan L. Spalding, Gary J. Patti
    Pages 1-15

  3. High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry

    • Kim A. Lagerborg, Jeramie D. Watrous, Susan Cheng, Mohit Jain
    Pages 17-35

  4. Measuring the Nutrient Metabolism of Adherent Cells in Culture

    • Martin P. Ogrodzinski, Shao Thing Teoh, Lei Yu, Deanna Broadwater, Elliot Ensink, Sophia Y. Lunt
    Pages 37-52

  5. 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells

    • Marit van Gorsel, Ilaria Elia, Sarah-Maria Fendt
    Pages 53-66

  6. Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice

    • Dorien Broekaert, Sarah-Maria Fendt
    Pages 67-82

  7. Measuring Human Lipid Metabolism Using Deuterium Labeling: In Vivo and In Vitro Protocols

    • Katherine E. Pinnick, Pippa J. Gunn, Leanne Hodson
    Pages 83-96

  8. Measuring Rates of ATP Synthesis

    • Matthew J. Bird, Silvia Radenkovic, Pieter Vermeersch, David Cassiman
    Pages 97-107

  9. Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay

    • Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, Oliver D. K. Maddocks
    Pages 109-119

  10. Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers

    • Koen Veys, Abdiel Alvarado-Diaz, Katrien De Bock
    Pages 121-136

  11. Determining Compartment-Specific Metabolic Fluxes

    • Yannic Nonnenmacher, Roberta Palorini, Karsten Hiller
    Pages 137-149

  12. Determining the Impact of Metabolic Nutrients on Autophagy

    • Jessica D. Guillaume, Stephanie L. Celano, Katie R. Martin, Jeffrey P. MacKeigan
    Pages 151-162

  13. Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism

    • Franziska Püschel, Cristina Muñoz-Pinedo
    Pages 163-172

  14. Determining Macrophage Polarization upon Metabolic Perturbation

    • Pu-Ste Liu, Ping-Chih Ho
    Pages 173-186

  15. Assessing the Impact of the Nutrient Microenvironment on the Metabolism of Effector CD8+ T Cells

    • Juan Fernández-García, Sarah-Maria Fendt
    Pages 187-216

  16. Development of Patient-Derived Tumor Xenograft Models

    • Daniela Annibali, Eleonora Leucci, Els Hermans, Frédéric Amant
    Pages 217-225

  17. Imaging Glioma Progression by Intravital Microscopy

    • Fabio Stanchi, Ken Matsumoto, Holger Gerhardt
    Pages 227-243

  18. Lipectomizing Mice for Applications in Metabolism

    • Debrup Chakraborty, Jamie J. Bernard
    Pages 245-250

  19. Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants

    • Wen-Rong Lie, Derek F. Amanatullah, Bonnie L. King
    Pages 251-262

  20. Determining the Intracellular Organization of Organelles

    • Bruno Latgé, Kristine Schauer
    Pages 263-278
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Keywords

About this book

This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches.  Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level.

Editors and Affiliations

  • Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium

    Sarah-Maria Fendt

  • Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, USA

    Sophia Y. Lunt

Bibliographic Information

  • Book Title: Metabolic Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Sarah-Maria Fendt, Sophia Y. Lunt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8769-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8768-9Published: 13 October 2018

  • Softcover ISBN: 978-1-4939-9387-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8769-6Published: 12 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 294

  • Number of Illustrations: 14 b/w illustrations, 53 illustrations in colour

  • Topics: Cell Biology

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