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Microarray Technology

Methods and Applications

  • Book
  • © 2016

Overview

Editors:
  1. Paul C.H. Li

    1. Department of Chemistry, Simon Fraser University, Burnaby, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Abootaleb Sedighi

    1. Dept. of Chemistry, Simon Fraser University, Burnaby, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Lin Wang

    1. Department of Biomedical Engineering, University of British Columbia, Vancouver, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1368)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Overview of Microarray Technology

      • Paul C. H. Li
      Pages 3-4

  3. Advances on Techniques in Microarray Technology

    1. Front Matter

      Pages 5-5
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    2. Surface Functionalization for Immobilization of Probes on Microarrays

      • C. Desmet, C. A. Marquette
      Pages 7-23

    3. The “Clickable” Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays

      • Tomasz Ratajczak, Barbara Uszczyńska, Emilia Frydrych-Tomczak, Marcin K. Chmielewski
      Pages 25-36

    4. Microarray Developed on Plastic Substrates

      • María-José Bañuls, Sergi B. Morais, Luis A. Tortajada-Genaro, Ángel Maquieira
      Pages 37-51

    5. Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray

      • Ryo Iizuka, Taro Ueno, Takashi Funatsu
      Pages 53-65

    6. Probe Design Strategies for Oligonucleotide Microarrays

      • Nicolas Parisot, Eric Peyretaillade, Eric Dugat-Bony, Jérémie Denonfoux, Antoine Mahul, Pierre Peyret
      Pages 67-82

  4. Applications of Microarray Technology to Nucleic Acid Assays

    1. Front Matter

      Pages 83-83
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    2. Analyzing Illumina Gene Expression Microarray Data Obtained From Human Whole Blood Cell and Blood Monocyte Samples

      • Alexander Teumer, Claudia Schurmann, Arne Schillert, Katharina Schramm, Andreas Ziegler, Holger Prokisch
      Pages 85-97

    3. Microarray Technology and Its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers

      • Hirotaka Konishi, Daisuke Ichikawa, Tomohiro Arita, Eigo Otsuji
      Pages 99-109

    4. Application of DNA Microarray to Clinical Diagnostics

      • Ankita Patel, Sau W. Cheung
      Pages 111-132

    5. High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device

      • Abootaleb Sedighi, Paul C. H. Li
      Pages 133-141

    6. Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies

      • Annalisa Lonetti, Maria Chiara Fontana, Giovanni Martinelli, Ilaria Iacobucci
      Pages 143-159

    7. DNA Microarray-Based Diagnostics

      • Mahsa Gharibi Marzancola, Abootaleb Sedighi, Paul C. H. Li
      Pages 161-178

  5. Applications of Microarray Technology to Other Biological Assays

    1. Front Matter

      Pages 179-179
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    2. High-Throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays

      • Liqian Gao, Su Seong Lee, Jun Chen, Hongyan Sun, Yuliang Zhao, Zhifang Chai et al.
      Pages 181-196

    3. Nanotechnology in the Fabrication of Protein Microarrays

      • Manuel Fuentes, Paula Díez, Juan Casado-Vela
      Pages 197-208

    4. Epitope Mapping Using Peptide Microarray in Autoantibody Profiling

      • Sebastian Henkel, Robert Wellhausen, Dirk Woitalla, Katrin Marcus, Caroline May
      Pages 209-224
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Keywords

About this book

This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Chemistry, Simon Fraser University, Burnaby, Canada

    Paul C.H. Li

  • Dept. of Chemistry, Simon Fraser University, Burnaby, Canada

    Abootaleb Sedighi

  • Department of Biomedical Engineering, University of British Columbia, Vancouver, Canada

    Lin Wang

Bibliographic Information

  • Book Title: Microarray Technology

  • Book Subtitle: Methods and Applications

  • Editors: Paul C.H. Li, Abootaleb Sedighi, Lin Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3136-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3135-4Published: 28 November 2015

  • Softcover ISBN: 978-1-4939-4907-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3136-1Published: 27 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 294

  • Topics: Human Genetics, Microarrays

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