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Methods in Molecular Biology

Axon Growth and Regeneration

Methods and Protocols

Editors: Murray, Andrew J. (Ed.)

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  • Includes cutting-edge methods and protocols involving axon growth and regeneration
  • Provides step-by-step detail essential for reproducible results in the lab
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-0777-9
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About this book

Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors and methods for quantifying outgrowth. The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery. As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field.

Reviews

From the reviews:

“This is a ‘how to’ manual of protocols, parameters, and cell biology methods for research studies of neurite outgrowth assays. … The authorship is very good and interesting to neurobiologists, cell culture experts, and neurology-neurosurgery researchers. … I highly recommend this book for all neurobiologists with interests in cell and slice physiology.” (Joseph J. Grenier, Amazon.com, June, 2014)

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Axon Regeneration: What Needs to Be Overcome?

    Pages 3-14

    Murray, Andrew J.

  • Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro

    Pages 17-27

    Akiyama, Hiroki (et al.)

  • The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance-Related Intracellular Signalling in Live Dorsal Root Ganglia Neuronal Growth Cones

    Pages 29-40

    Tucker, Steven J.

  • Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues

    Pages 43-56

    Kaplan, Andrew (et al.)

  • Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants

    Pages 57-71

    Buyens, Tom (et al.)

Buy this book

eBook £73.50
price for United Kingdom (gross)
  • ISBN 978-1-4939-0777-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover £139.99
price for United Kingdom (gross)
  • ISBN 978-1-4939-0776-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover £92.00
price for United Kingdom (gross)
  • ISBN 978-1-4939-4584-9
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Axon Growth and Regeneration
Book Subtitle
Methods and Protocols
Editors
  • Andrew J. Murray
Series Title
Methods in Molecular Biology
Series Volume
1162
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0777-9
DOI
10.1007/978-1-4939-0777-9
Hardcover ISBN
978-1-4939-0776-2
Softcover ISBN
978-1-4939-4584-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 253
Number of Illustrations
13 b/w illustrations, 53 illustrations in colour
Topics

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