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Bone Marrow Environment

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Marion Espéli

    1. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

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  2. Karl Balabanian

    1. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2308)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
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  2. Cell Culture and In Vitro Functionnal Assays

    1. Front Matter

      Pages 1-1
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    2. Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells

      • Valeria Bisio, Marion Espéli, Karl Balabanian, Adrienne Anginot
      Pages 3-20

    3. Differentiation and Phenotyping of Murine Osteoclasts from Bone Marrow Progenitors, Monocytes, and Dendritic Cells

      • Julia Halper, Maria-Bernadette Madel, Claudine Blin-Wakkach
      Pages 21-34

    4. Culture, Expansion and Differentiation of Mouse Bone-Derived Mesenchymal Stromal Cells

      • Zeina Abou Nader, Marion Espéli, Karl Balabanian, Julia P. Lemos
      Pages 35-46

    5. Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells

      • Vincent Rondeau, Marion Espéli, Karl Balabanian
      Pages 47-58

    6. In Vitro Analysis of Energy Metabolism in Bone-Marrow Mesenchymal Stromal Cells

      • Jérôme Bourgeais, Olivier Hérault
      Pages 59-70

  3. Flow Cytometry-Based Analysis of the Bone Marrow Cellular Compartments

    1. Front Matter

      Pages 71-71
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    2. Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells

      • Julien M. P. Grenier, Marjorie C. Delahaye, Stéphane J. C. Mancini, Michel Aurrand-Lions
      Pages 73-81

    3. Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment

      • Yuki Matsushita, Wanida Ono, Noriaki Ono
      Pages 83-94

    4. Immunophenotyping of the Medullary B Cell Compartment In Mouse Models

      • Amélie Bonaud, Karl Balabanian, Marion Espéli
      Pages 95-105

    5. Metabolic Analysis of Mouse Hematopoietic Stem and Progenitor Cells

      • Liang He, Amina Aouida, Amira Mehtar, Houda Haouas, Fawzia Louache
      Pages 107-115

  4. Imaging of the Bone Marrow

    1. Front Matter

      Pages 117-117
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    2. Multicolor Immunofluorescence Staining of Paraffin-Embedded Human Bone Marrow Sections

      • Francesca M. Panvini, Simone Pacini, Stefano Mazzoni, Simón Méndez-Ferrer
      Pages 119-126

    3. 3D Microscopy of Murine Bone Marrow Hematopoietic Tissues

      • YeVin Mun, César Nombela-Arrieta
      Pages 127-138

    4. Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrow

      • Stephan Holtkamp, Christoph Scheiermann
      Pages 139-150

    5. Intrafemoral Delivery of Hematopoietic Progenitors

      • Maximilien Evrard, Immanuel Kwok, Lai Guan Ng
      Pages 151-161

    6. Imaging of Bone Marrow Plasma Cells and of Their Niches

      • Carolin Ulbricht, Raluca A. Niesner, Anja E. Hauser
      Pages 163-176

    7. Intravital Imaging of Bone Marrow Microenvironment in the Mouse Calvaria and Tibia

      • Changming Shih, Leonard Tan, Jackson Liang Yao Li, Yingrou Tan, Hui Cheng, Lai Guan Ng
      Pages 177-202

    8. Intravital Imaging of Bone Marrow Niches

      • Myriam L. R. Haltalli, Cristina Lo Celso
      Pages 203-222
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Keywords

About this book

This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years.

Editors and Affiliations

  • Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Marion Espéli, Karl Balabanian

Bibliographic Information

  • Book Title: Bone Marrow Environment

  • Book Subtitle: Methods and Protocols

  • Editors: Marion Espéli, Karl Balabanian

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1425-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1424-2Published: 01 June 2021

  • Softcover ISBN: 978-1-0716-1427-3Published: 02 June 2022

  • eBook ISBN: 978-1-0716-1425-9Published: 31 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 340

  • Number of Illustrations: 4 b/w illustrations, 67 illustrations in colour

  • Topics: Biotechnology, Stem Cells

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